Human PD-L1 ELISA Kit

$495.00

The Eagle Biosciences Human PD-L1/B7-H1 ELISA Kit is intended for the quantitation of Human CD274 concentrations in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). The Eagle Biosciences Human PD-L1/B7-H1 ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

Human PD-L1 ELISA Kit

The Human PD-L1 ELISA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: <12pg/ml
Standard Range: 62.5pg/ml-4000pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: Cell culture supernates, Cell lysates, Serum and Plasma
Sample Size: 100 µl
Alternative Name: B7-H1, Programmed death-ligand 1, CD274, B7 homolog 1


Assay Principle

The Human CD274 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CD274 with a 96-well strip plate that is pre-coated with antibody specific for CD274. The detection antibody is a biotinylated antibody specific for CD274. The capture antibody is polyclonal antibody from goat, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human CD274 with immunogen: Expression system for standard: NSO; Immunogen sequence: F19-R238. The kit is analytically validated with ready to use reagents. To measure Human CD274, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human CD274 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Human CD274 in the sample.


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Additional Information

Assay Background


Programmed death-ligand 1 (PD-L1), also known as cluster of differentiation 274 (CD274) or B7 homolog 1 (B7-H1), is a protein that in humans is encoded by the CD274 gene. PD-L1 is a 40kDa type 1 transmembrane protein that has been speculated to play a major role in suppressing the immune system during particular events such as pregnancy, tissue allografts, autoimmune disease and other disease states such as hepatitis. Normally the immune system reacts to foreign antigens where there is some accumulation in the lymph nodes or spleen which triggers a proliferation of antigen-specific CD8+ T cell. The formation of PD-1 receptor / PD-L1 or B7.1 receptor /PD-L1 ligand complex transmits an inhibitory signal which reduces the proliferation of these CD8+ T cells at the lymph nodes and supplementary to that PD-1 is also able to control the accumulation of foreign antigen specific T cells in the lymph nodes through apoptosis which is further mediated by a lower regulation of the gene Bcl-2.

Typical Standard Curve


Human PD-L1 ELISA Kit

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