Human [Leu60] IGF-I

$170.00

The Eagle Bioscience’s Human [Leu60] IGF-I is for research use only and not to be used for diagnostic procedures.

SKU: ABU100 Categories: ,

Human [Leu60] IGF-I

The Eagle Bioscience’s Human [Leu60] IGF-I is for research use only and not to be used for diagnostic procedures.


Product Specifications

Source:

IGF-I is produced in E.coli using a patented expression system and the IGF-I is correctly folded and purified by several chromatography steps to yield Receptor Grade IGF-I.

Description:

Human [Leu60] insulin-like Growth Factor-I ([Leu60] IGF-I) is an analogue of human IGF-I which has a Leucine substitution in place of Tyrosine at position 60 of the human IGF-I sequence. [Leu60]IGF-I has normal binding to acid-stripped human serum binding proteins but has reduced binding to the type 1 IGF receptor and to the insulin receptor compared to IGF-I. It is a useful analogue for studying the relative effects of IGF-I through the type 1 receptor and IGF binding proteins. 

  • Purity: >95 % (as determined by HPLC analysis)
  • Quantity: 100 µg
  • Molecular Weight: 7599 daltons – confirmed by Mass Spectrometry
  • N-terminal sequence analysis of recombinant product: Analysis of 5 residues
  • Biological Activity: Stimulation of protein synthesis in rat L6 myoblasts (ED50 >2000 ng/ml) IGF type 1 receptor binding assay (ED50 > 100 ng/ml) IGF binding protein assay: ED50 < 10 ng/ml)
  • Endotoxin (LAL): <0.1 EU/ug
  • Storage/Stability: At least 2 years at 2-4°C (lyophilized)
  • Appearance: White Powder
  • State: Dried from 0.1 M acetic acid under dry nitrogen at a slight vacuum (-25kPa)

Related Products

Rodent IGF-I ELISA Assay
Human Arg3 IGF-1 Biotin
Biotinylated Human Des IGF-1

Documents

Product Manual


Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

Product Citations