Human Influenza Viruses IgG ELISA Assay Kit

$350.00

Human Influenza Viruses IgG ELISA is used as an analytical tool for quantitative determination of Influenza Viruses IgG in human serum, plasma and cell culture supernatant. The Human Influenza Viruses IgG ELISA Assay Kit is for research use only and not to be used for diagnostic procedures.

Human Influenza Viruses IgG ELISA Assay Kit

The Human Influenza Viruses IgG ELISA Assay Kit is For Research Use Only

Size: 96 wells
Standard Range: 4-64 pg/ml
Sensitivity: 2 pg/ml
Incubation Time: min 1 hour 10 minutes
Sample Type: Serum or Plasma
Sample Size: 40 µL


Assay Principle

This is sandwich enzyme-linked immunosorbent assay (ELISA) to assay the level of Influenza Viruses IgG in samples. Addition of standard or sample to microtiter well which is pre-coated with Human Influenza Viruses IgG monoclonal antibody and addition of biotin labeled Human Influenza Viruses IgG antibody, followed by addition of HRP conjugate to form immune complex. Unbound HRP conjugate will get removed by washing step after incubation. Then addition of Substrate A and B, develops blue color during incubation period and reaction will get stop after addition of stop solution with development of yellow color. The concentration of the Human Influenza Viruses IgG of sample is directly proportional to the yellow color developed in well and will be positively correlated.


Sample Preparation and Storage

Specimens should be clear and non-hemolyzed. Samples should be run at a number of dilutions to ensure accurate quantitation.

Serum: Use a serum separator tube and allow clotting for 30 minutes, then centrifuge for 10 minutes at 1000 x g. Remove serum layer and assay immediately or store serum samples at -20°C. Avoid repeated freeze/thaw cycles.

Plasma: Collect blood sample in a citrate, heparin or EDTA containing tube. Centrifuge for 10 minutes at 1000 x g within 30 minutes of collection. Assay immediately or store plasma samples at < -20°C. Avoid repeated freeze/thaw cycles.

Note: Grossly hemolyzed samples are not suitable for use in this assay.


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Additional Information

Assay Background


The influenza infection is an acute feverish virus infection of the respiratory tract. The infection mostly results from a droplet infection and appears as an epidemic or pandemy. The virus and its toxin can lead to a serious inflammation of the bronchial mucosa and a damage of the vessels. After incubation time of 1 to 3 days the symptoms appear suddenly: Followed by a fast raise of temperature, often accompanied by shivering, the leading symptom of catarrhal inflammation appears, contributing to the clinical course of painful dry cough, tracheitis, laryngitis and frequently a rhinitis and conjunctivitis. The influenza infection is an acute feverish virus infection of the respiratory tract. The infection mostly results from a droplet infection and appears as an epidemic or pandemy. The virus and its toxin can lead to a serious inflammation of the bronchial mucosa and a damage of the vessels. After incubation time of 1 to 3 days the symptoms appear suddenly: Followed by a fast raise of temperature, often accompanied by shivering, the leading symptom of catarrhal inflammation appears, contributing to the clinical course of painful dry cough, tracheitis, laryngitis and frequently a rhinitis and conjunctivitis.

Documents

Product Manual


Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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