Human Influenza Viruses B ELISA Assay Kit


Human Influenza Viruses B ELISA Assay Kit is used as an analytical tool for quantitative determination of FLU B ELISA in human serum, plasma and cell culture supernatant. The Human Influenza Viruses B ELISA Assay Kit is for research use only and not to be used for diagnostic procedures.

Human Influenza Viruses B ELISA Assay Kit

The Human Influenza Viruses B ELISA Assay Kit is For Research Use Only

Size: 96 wells
Standard Range: 62.5-4000 pg/ml
Sensitivity: 62.5 pg/ml
Incubation Time: min 1 hour 10 minutes
Sample Type: Serum or Plasma
Sample Size: 40 µL

Assay Principle

This is sandwich enzyme-linked immunosorbent assay (ELISA) to assay the level of Human FLU B in samples. Addition of standard or sample to microtiter well which is pre-coated with Human FLU B monoclonal antibody and addition of biotin labeled Human FLU B antibody, followed by addition of HRP conjugate to form immune complex. Unbound HRP conjugate will get removed by washing step after incubation. Then addition of Substrate A and B, develops blue color during incubation period and reaction will get stop after addition of stop solution with development of yellow color. The concentration of the Human Influenza Viruses B of sample is directly proportional to the yellow color developed in well and will be positively correlated.

Sample Preparation and Storage

Specimens should be clear and non-hemolyzed. Samples should be run at a number of dilutions to ensure accurate quantitation.

Serum: Use a serum separator tube and allow clotting for 30 minutes, then centrifuge for 10 minutes at 1000 x g. Remove serum layer and assay immediately or store serum samples at < -20°C. Avoid repeated freeze/thaw cycles.

Plasma: Collect blood sample in a citrate, heparin or EDTA containing tube. Centrifuge for 10 minutes at 1000 x g within 30 minutes of collection. Assay immediately or store plasma samples at < -20°C. Avoid repeated freeze/thaw cycles.

Note: Grossly hemolyzed samples are not suitable for use in this assay.

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Additional Information

Assay Background

Influenza B virus is the only species in the genus Betainfluenzavirus in the virus family Orthomyxoviridae. Influenza B virus is only known to infect humans and seals with influenza. This limited host range is apparently responsible for the lack of associated influenza pandemics in contrast with those caused by the morphologically similar influenza A virus as both mutate by both antigenic drift and reassortment. There are two known circulating lineages of Influenza B virus based on the antigenic properties of the surface glycoprotein hemagglutinin.

Package Inserts

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