Human Influenza Viruses B ELISA Assay Kit
The Human Influenza Viruses B ELISA Assay Kit is For Research Use Only
Size: 96 wells
Standard Range: 62.5-4000 pg/ml
Sensitivity: 62.5 pg/ml
Incubation Time: min 1 hour 10 minutes
Sample Type: Serum or Plasma
Sample Size: 40 µL
Assay Principle
This is sandwich enzyme-linked immunosorbent assay (ELISA) to assay the level of Human FLU B in samples. Addition of standard or sample to microtiter well which is pre-coated with Human FLU B monoclonal antibody and addition of biotin labeled Human FLU B antibody, followed by addition of HRP conjugate to form immune complex. Unbound HRP conjugate will get removed by washing step after incubation. Then addition of Substrate A and B, develops blue color during incubation period and reaction will get stop after addition of stop solution with development of yellow color. The concentration of the Human Influenza Viruses B of sample is directly proportional to the yellow color developed in well and will be positively correlated.
Sample Preparation and Storage
Specimens should be clear and non-hemolyzed. Samples should be run at a number of dilutions to ensure accurate quantitation.
Serum: Use a serum separator tube and allow clotting for 30 minutes, then centrifuge for 10 minutes at 1000 x g. Remove serum layer and assay immediately or store serum samples at < -20°C. Avoid repeated freeze/thaw cycles.
Plasma: Collect blood sample in a citrate, heparin or EDTA containing tube. Centrifuge for 10 minutes at 1000 x g within 30 minutes of collection. Assay immediately or store plasma samples at < -20°C. Avoid repeated freeze/thaw cycles.
Note: Grossly hemolyzed samples are not suitable for use in this assay.
