Human IGF-I (Media Grade)
The Eagle Bioscience’s Human IGF-I (Media Grade) is for research use only and not to be used for diagnostic procedures.
IGF-I is produced in E.coli using a patented expression system and the IGF-I is correctly folded and purified by several chromatography steps to yield Receptor Grade IGF-I.
Human insulin-like growth factor-I (IGF-I) is a single chain 70 amino acid polypeptide that shares structural homology with insulin and human IGF-II. IGF-I plays a critical role in growth, differentiation, and development via autocrine, paracrine and endocrine mechanisms. It is a mitogen and also has effects on cell migration and differentiation. IGF-I binds to the type I IGF receptor and most of its actions are probably mediated through inducing signal transduction via this receptor. IGF-I also binds to each of the six IGF binding proteins (IGFBPs). This binding also regulates the biological function of IGF-I. IGF-I is highly conserved across species which suggests an important role for the peptide. Human IGF-I is present in high concentrations in normal adult human serum in a complex with IGFBP-3 and the acid-labile subunit (ALS) and mediates many of the actions of Growth Hormone. IGF-I is a regulator of metabolism and is also implicated in the pathophysiology of cancer, diabetes, and obesity.
- Purity: >85 % (as determined by HPLC analysis)
- Quantity: 100 µg
- Molecular Weight: 7649 ± 2 Daltons- confirmed by Mass Spectrometry
- N-terminal sequence analysis of recombinant product: 5 residues >95% single sequence
- Biological Activity: Proliferation of Chinese hamster ovary (CHO) cells (EC50 < 350 ng/ml)
- Endotoxin (LAL): <0.1 EU/ug
- Storage/Stability: At least 2 years at 2-8°C (lyophilized)
- Appearance: White Powder
- State: Dried from 0.1 M acetic acid under dry nitrogen at a slight vacuum (-25kPa)