Receptor Grade IGF-I Recombinant Protein

$670.00

The Eagle Biosciences Human IGF-I Recombinant Protein (Receptor Grade) is for research use only.

SKU: CU100-100 Category:

Receptor Grade IGF-I Recombinant Protein

The Receptor Grade IGF-I Recombinant Protein is For Research Use Only

Aliquot: 100 µg
Storage: 4°C for up to 2 years
Alternative Name: Human Insulin-Like Growth Factor-1 (Receptor Grade)


Product Specifications


Quantity:
100 µg
Description:
Insulin-like growth factor-I (IGF-I) is a 70 amino acid polypeptide that stimulates growth and differentiation in many cell types including rat L6 myoblasts.
Source:
IGF-I is produced in E. coli using a patented expression system and the IGF-I is correctly folded and purified by several chromatography steps to yield Receptor Grade IGF-I.

  • Purity: > 95% (as determined by HPLC and N-terminal sequence analysis)
  • Molecular Weight: 7649 daltons – confirmed by Mass Spectrometry
  • N-terminal sequence analysis of recombinant product: 5 residues >95% single sequence
  • Biological Activity: (stimulation of protein synthesis in rat L6 myoblasts)   ED50  <30 ng/ml
  • Endotoxin (LAL): <0.1 EU/ugStorage/Stability: At least 2 years at 2-4°C (lyophilized)
  • Appearance: White Powder
  • State: Dried from 0.1 M acetic acid: under dry nitrogen at slight vacuum (-25kPa)

Related Products

Biotinylated Human Des IGF-1
Human Arg3 IGF-1 Biotin
Biotin Leu24 IGF-1
Product Developed and Manufactured in Germany by ibt – immunological and biochemical testsystems GmbH

Publications

References


  1. Granata et al. (2007): Insulin-like growth factor binding protein-3 induces angiogenesis through IGF-I- and SphK1-dependent mechanisms. Journal of Thrombosis and Haemostasis. Volume 5, Issue 4, Pages 835-845.
  2. Ciucci F, Putignano E, Baroncelli L, Landi S, Berardi N, et al. (2007) Insulin-Like Growth Factor 1 (IGF-1) Mediates the Effects of Enriched Environment (EE) on Visual Cortical Development. PLoS ONE 2(5): e475 doi:10.1371/journal.pone.0000475

Citations

Jiang, MS;Yang, X;Esposito, D;Nelson, E;Yuan, J;Hopkins, RF;Broadt, T;Xiao, Z;Colantonio, S;Prieto, DA;Welch, AR;Creekmore, SP;Mitra, G;Zhu, J.  (2015) Mammalian cell transient expression, non-affinity purification, and characterization of human recombinant IGFBP7, an IGF-1 targeting therapeutic protein

Product Citations