Human CD54 ELISA Assay
The Human CD54 ELISA Assay is For Research Use Only
Size: 1×96 wells
Sensitivity: 0.1 ng/ml
Dynamic Range: 0.25 ng/ml – 8 ng/ml
Incubation Time: 1h 40min
Sample Type: Serum, Plasma, Cell Culture
Sample Size: 100 µl
Alternative Name: ICAM-1 ELISA, Intercellular Adhesion Molecule 1 ELISA
Specimen collection, processing & storage
Cell culture supernatants, serum, plasma or other biological samples will be suitable for use in the assay. Remove serum from the clot or red cells, respectively, as soon as possible after clotting and separation.
Cell culture supernatants:
Remove particulates and aggregates by spinning at approximately 1000 x g for 10 min.
Avoid any unintentional stimulation of the cells by the procedure. Use pyrogen/endotoxin free collecting tubes. Serum should be removed rapidly and carefully from the red cells after clothing. For that, after clothing, centrifuge at approximately 1000 x g for 10 min and remove serum.
EDTA, citrate and heparin plasma can be assayed. Spin samples at 1000 x g for 30 min to remove particulates. Harvest plasma.
If not analyzed shortly after collection, samples should be aliquoted (250-500μl) to avoid repeated freeze-thaw cycles and stored frozen at –70°C. Avoid multiple freeze-thaw cycles of frozen specimens.
ICAM-1 (InterCellular Adhesion Molecule) or CD54 is a protein encoded by the human ICAM1 gene located on human chromosome 19. It is present on the surface of endothelial cells and lymphocytes. It can serve as a receptor for viruses of the genus rhinovirus.
The protein encoded by this gene is a type of continuous intercellular adhesion molecule present at low concentrations in leukocyte membranes and endothelial cells. During cytokine stimulation, the concentrations increase considerably. When activated, leukocytes bind to endothelial cells via ICAM-1 / LFA-1 and then transmigrate into tissues.
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