Human CD102 sICAM-2 ELISA Assay
The Human CD102 sICAM-2 ELISA Assay is For Research Use Only
Size: 1×96 wells
Sensitivity: 0.2 U/ml
Dynamic Range: 0.75 U/ml – 24 U/ml
Incubation Time: 1h 40min
Sample Type: Serum, Plasma, Cell Culture
Sample Size: 100 µl
Alternative Name: Soluble Intercellular Adhesion Molecule 2
Specimen collection, processing & storage
Cell culture supernatants, serum, plasma or other biological samples will be suitable for use in the assay. Remove serum from the clot or red cells, respectively, as soon as possible after clotting and separation.
Cell culture supernatants:
Remove particulates and aggregates by spinning at approximately 1000 x g for 10 min.
Serum:
Avoid any unintentional stimulation of the cells by the procedure. Use pyrogen/endotoxin free collecting tubes. Serum should be removed rapidly and carefully from the red cells after clotting. For that, after clotting, centrifuge at approximately 1000 x g for 10 min and remove serum. Before testing, human serum samples have to be diluted 20 times in standard buffer diluent.
Plasma:
EDTA, citrate and heparin plasma can be assayed. Spin samples at 1000 x g for 30 min to remove particulates. Harvest plasma.
Storage:
If not analyzed shortly after collection, samples should be aliquoted (250-500μl) to avoid repeated freeze-thaw cycles and stored frozen at –70°C. Avoid multiple freeze-thaw cycles of frozen specimens.
Assay Background
The protein encoded by this gene belongs to the family of intercellular adhesion molecules (ICAM). All ICAM proteins are type I transmembrane glycoproteins, contain 2 to 9 immunoglobulin type C2 domains, and bind to LFA-1 leukocyte adhesion protein.
ICAM-2 molecules regulate the adhesion of spermatids to the Sertoli cell on the apical side of the blood-testis barrier (to light), thus playing a major role in spermatogenesis.
This protein may also play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It is involved in important adhesive interactions for the antigen-specific immune response, NK-mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.
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