Human Beta 2-Microglobulin ELISA
The Human Beta 2-Microglobulin ELISA is For Research Use Only
Size: 1×96 wells
Sensitivity: 0.822 ng/ml
Standard Range: 3.125 – 100 ng/ml
Incubation Time: 130 minutes
Sample Type: Biological Fluids
Sample Size: 100 µl
Alternative Name:B2M ELISA
Assay Principle
The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Beta 2-Microglobulin present in samples reacts with the anti- Beta 2-Microglobulin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-B2M antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound B2M. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of B2M in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of B2M in the test sample. The quantity of B2M in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Specimen Collection and Handling
Blood should be collected by venipuncture. The serum should be separated from the cells after clot formation by centrifugation. For plasma samples, blood should be collected into a container with an anticoagulant and then centrifuged. Care should be taken to minimize hemolysis, excessive hemolysis can impact your results. Assay immediately or aliquotand store samples at -20C. Avoid repeated freeze thaw cycles.
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