High Sensitive Rat Insulin ELISA Assay Kit


The High Sensitive Rat Insulin ELISA Assay Kit is designed for quantification of rat insulin in serum, plasma, and fluid. The Eagle Biosciences High Sensitive Rat Insulin ELISA Assay Kit is for research use only and not to be used for diagnostic procedures.

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High Sensitive Rat Insulin ELISA Assay Kit

The High Sensitive Rat Insuslin ELISA Assay Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: 0.2ng/mL
Standard Range: 0.2ng/mL-7ng/mL
Incubation Time: 1 hour 45 minutes
Sample Type: Serum, plasma, and fluid
Sample Size: 10 µl

Assay Principle

The High Sensitive Rat Insulin assay is a two-site ELISA. The micro-plate is pre-coated with a monoclonal antibody against insulin. Standards and samples are added into the wells and co-incubated with a monoclonal antibody conjugated to horseradish peroxidase (HRP) enzyme. After wash step to remove any unbound substances, TMB substrate is added and color develops in proportion to the amount of insulin bound initially. The assay is stopped and the optical density of the wells determined using a micro-plate reader. Since the increases in absorbance are directly proportional to the amount of captured insulin, the unknown sample concentration can be interpolated from a reference curve included in each assay.

Sample Handlinge

No dilution of the sample is required in this assay, if a sample has an insulin
level greater than the highest standard, the sample should be diluted with 0
ng/ml insulin standard solution and the assay should be repeated.

Related Products

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Human Insulin Biotinylated (Biotin-Insulin)

Additional Information

Typical Standard Curve

High Sensitive Rat Insulin ELISA Kit


Product Manual

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