High Sensitive IFN gamma ELISA Assay

High Sensitive IFN gamma ELISA Assay

$905.00

The High Sensitive IFN Gamma ELISA Assay Kit is intended for for the quantification of Human Interferon Gamma in serum, plasma, cell culture supernatants. The High Sensitive IFN Gamma ELISA Assay Kit is for research use only and should not be used for diagnostic procedures.

SKU: ARG80116 Categories: , , Tags: , ,

High Sensitive IFN gamma ELISA Assay

The High Sensitive IFN gamma ELISA Assay is For Research Use Only

Size: 1 x 96 wells
Sensitivity: 0.8 pg/ml
Dynamic Range: 1.56-100 pg/ml
Incubation Time: 4 hours
Sample Type: Serum, Plasma, Cell culture Supernatants
Sample Size: 100 µl

Assay Principle

The High Sensitive IFN Gamma ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for IFN gamma has been pre-coated onto a microtiter plate. Standards or samples are pipetted into the wells and any IFN gamma present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-conjugated antibody specific for IFN gamma is added to each well and incubate. Following a washing to remove unbound substances, streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After washing away any unbound antibody-enzyme reagent, a substrate solution (TMB) is added to the wells and color develops in proportion to the amount of IFN gamma bound in the initial step. The color development is stopped by the addition of acid and the intensity of the color is measured at a wavelength of 450nm ±2nm.The concentration of IFN gamma in the sample is then determined by comparing the O.D of samples to the standard curve.

Related Products

Swine IFN-gamma ELISA
Interferon Gamma ELISA Assay Kit

Additional Information

Assay Background

This gene encodes a member of the type II interferon family. The protein encoded is a soluble cytokine with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. Mutations in this gene are associated with aplastic anemia.

Assay Procedure

  1. Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal it.
  2. Add 100 μl of standards, samples and zero controls (standard diluent buffer) into wells. Incubate for 1.5 h at 36 °C.
  3. Aspirate each well and wash, repeating the process four times for a total five washes. Wash by filling each well with 1× Wash Buffer (350 μl) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating, decanting or blotting against clean paper towels.
  4. Add 100 μl 1X Antibody conjugate into each well. Cover wells and incubate for 1 hour at 36°C.
  5. Aspirate each well and wash as step 3.
  6. Add 100 μl of 1X HRP-Streptavidin solution to each well. Cover wells and incubate for 30 minutes at 36°C.
  7. Aspirate each well and wash as step 3.
  8. Add 100 μl of TMB Reagent to each well. Incubate for 15 minutes at 36°C in dark.
  9. Add 100 μl of Stop Solution to each well. The color of the solution should change from blue to yellow.
  10. Read the OD with a microplate reader at 450nm immediately.

Package Inserts

 

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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