High Sensitive CRP ELISA Assay Kit

$695.00

The Eagle Biosciences High Sensitive CRP ELISA Assay Kit is intended for the quantitative high sensitive determination of human C-Reactive Protein in serum and plasma. The Eagle Biosciences High Sensitive CRP ELISA Assay Kit is for research use only and should not be used for diagnostic procedures.

SKU: ARG80876 Categories: , ,

High Sensitive CRP ELISA Assay Kit:

For Research Use Only

Size: 1×96 wells
Sensitivity: 0.2 µg/mL
Dynamic Range:0.4-10 µg/mL
Incubation Time: ~1 hour
Sample Type: Serum, Plasma
Sample Size: 10 µL

Additional Information

Assay Background

C-Reactive Protein (CRP) is an acute-phase protein, produced exclusively in the liver. Interleukin- 6 is the mediator for the synthesis by the hepatocytes of CRP, a pentamer of approximately 120.000 Daltons. CRP is present in the serum of normal persons at concentrations ranging up to 5mg/l. A series of prospective studies provide consistent data documenting that mild elevation of baseline levels of CRP among apparently healthy individuals is associated with higher long term risk for future cardiovascular events. This predictive capacity of CRP is independent of traditional cardiovascular risk factors and offers a prognostic advantage over measurement of lipid alone. Inflammatory markers specifically CRP may help to identify those who would benefit most from these pharmacological intervention. CRP is the novel and evolving biomarker which provides a most useful predictive indicator for subsequent cardiovascular events. This test should not be used for assessment of acute inflammation but should be ordered to evaluate CVD (Cardiovascular Disease) risk in apparently healthy individuals who have not had recent infection or other serious illness.

Assay Principle

The Eagle Biosciences High Sensitive CRP assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for CRP has been pre-coated onto a microtiter plate. Standards or samples are pipetted into the wells and any CRP present is bound by the immobilized antibody. After washing away any unbound substances, an HRP-CRP antibody conjugate is added to each well and incubate. After washing away any unbound antibody-enzyme reagent, a substrate solution (TMB) is added to the wells and color develops in proportion to the amount of CRP bound in the initial step. The color development is stopped by the addition of acid and the intensity of the color is measured at a wavelength of 450 nm ±2 nm. The concentration of CRP in the sample is then determined by comparing the O.D of samples to the standard curve.

Assay Procedure

  1. Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal it.
  2. Add 100 µl of diluted standards and samples into each well.
  3. Incubate for 30 minutes at RT.
  4. Aspirate each well and wash, repeating the process 2 times for a total 3 washes. Wash by filling each well with wash buffer (350 μl) using a squirt bottle, manifold dispenser, or autowasher. Keep the wash buffer in the well for 1-2 min. Complete removal of liquid at each is essential to good performance. After the last wash, remove any remaining distilled water by aspirating, decanting or blotting against clean paper towels.
  5. Add 100 µl HRP-antibody conjugate into each well. Incubate for 30 minutes at RT.
  6. Aspirate and wash wells as step 4.
  7. Add 100 μl of TMB Substrate to each well. Incubate for 10 minutes at room temperature in dark.
  8. Add 50 μl Stop solution into each well.
  9. Read the OD with a microplate reader at 450 nm immediately.

Manual

Product Manual