High Five (H5) HCP ELISA Assay Kit
The High Five (H5) HCP ELISA Assay Kit is For Research Use Only
Size: 1 x 96 wells
Calibrator Range: 3 – 100 ng/mL
Sensitivity: 0.188 ng/mL
Sample Size: 100 uL
Sample Type: Cell Culture Supernatant and other Biological Preparations
Incubation Time: 2 hours 15 minutes
Assay Background
High Five cells (sometimes referred to as High-Five or Hi5) represent a safe, effective and inexpensive platform for protein production. Indeed, their remarkable ability to produce very large amounts of recombinant proteins – such as diagnostic reagents and recombinant vaccines – remains unmatched. The High Five cells were originally isolated from insects in BTI’s Granados lab in the late 1980’s. The baculovirus expression vector system (BEVS) in insect cells offers highly scalable and inexpensive protein production. High Five cells and sub-clones have been reported to produce 2-10x high levels of recombinant proteins compared to Sf9/Sf21 insect cells. BTI is the sole proprietor of High Five cells and related sub-clones. (BTI; Boyce Thomson Institute, NY, USA). The H5 (High Five) HCP ELISA kit is designed to quantitatively measure HCPs contamination in pharmaceutical products manufactured using the High Five Cells expression systems.
Assay Principle
The method employs sandwich ELISA technique. Monoclonal antibodies are pre-coated onto microwells. Samples and standards are pipetted into microwells and H5 HCP present in the sample are bound by the antibodies. HRP conjugated antibody is added and incubated to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of H5 HCP in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
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