H. pylori Antigen Quantitative ELISA Assay Kit

$460.00

The Eagle Biosciences H. pylori Antigen Quantitative ELISA Assay Kit is intended for use in the quantitative determination of H. pylori antigen in fecal or stool samples. The H. pylori ELISA Assay Kit is for research use only and not intended for diagnostic procedures.

SKU: HPL35-K01 Categories: , ,

H. pylori Antigen Quantitative ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 0.165 ng/mL
Dynamic Range: 0.19 – 150 ng/mL
Incubation Time: 2 hours
Sample Type: Stool

Controls Included

Product Developed and Manufactured in the USA

Additional Information

Assay Background


H. pylori (previously known as Campylobacter pyloridis) is a type of bacteria that infects the stomach and is a common cause of peptic ulcers. H. pylori bacteria can be passed from person to person through direct contact with saliva, vomit or fecal matter. H. pylori can also be spread through contaminated food or water. The infection is normally acquired during childhood. H. pylori usually goes undiagnosed until symptoms of a peptic ulcer occur. H. pylori infection is quite common and is present in about half the people in the world.

Assay Principle


The Eagle Biosciences Helicobacter pylori Antigen ELISA Assay Kit “sandwich” ELISA is designed, developed and produced for the quantitative measurement of H. pylori antigen in stool specimen. The assay utilizes the microplate-based enzyme immunoassay technique by coating highly purified antibody onto the wall of microtiter wells.

Assay calibrators and fecal specimen are added to microtiter wells of microplate that was coated with a highly purified monoclonal H. pylori antibody on its wall. During the assay, the H. pylori antigen will be bound to the antibody coated plate after an incubation period. The unbound material is washed away and another HRP-conjugated monoclonal antibody which specifically recognizes the protein of H. pylori is added for further immunoreactions. After an incubation period, the immunocomplex of “H. pylori Antibody – H. pylori Antigen – HRP-conjugated Anti-H. pylori Tracer Antibody” is formed if H. pylori antigen is present in the test sample. The unbound tracer antibody and other proteins in buffer matrix are removed in the subsequent washing step. HRP conjugated tracer antibody bound to the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the tracer antibody bound to H. pylori proteins captured on the wall of each microtiter well is directly proportional to the amount of H. pylori antigen level in each test specimen.

Typical Standard Curve


H. pylori Antigen Quantitative ELISA Assay Kit Standard Curve

Specificity


The Fecal H. pylori Ag ELISA Assay does not cross react to the following organisms: Cryptosoridium parvum, Giardia lamblia, rotavirus and adenovirus.

Manual

Product Manual