GLP-1 Solid Phase Sample Extraction Kit

GLP-1 Solid Phase Sample Extraction Kit

GLP-1 Solid Phase Sample Extraction Kit is developed and manufactured in the USA

This Kit is intended for use with the Eagle Biosciences, Inc. GLP-1 Total ELISA Assay and the Mouse Rat GLP-1 Active (7-36) ELISA

Size: Extracts 50 specimens

Assay Background

Immunoassays were developed for measuring serum GLP-1 level. However, many studies have found that the direct measurement of GLP-1 concentration in serum generates unreliable test results, which are not meaningful clinically and do not correlate at all among multiple immunoassays. Previous studies have suspected that there is an unknown matrix effect for measuring GLP-1 in serum sample by using immunoassays. Dai, et al. reported that solid phase extraction of serum sample before GLP-1 immunoassay eliminates the matrix effect and gives excellent GLP-1 test results, which include (1) clinical meaningful fast/fed GLP-1 serum concentration from normal donors and (2) excellent correlation among three different immunoassays for GLP-1 in serum. Therefore, it is essential to perform a sample extraction before assaying GLP-1.
There are several extraction methods available. Most methods require expensive and special laboratory equipment, such as a vacuum centrifuge. This product provides a well-validated solid phase GLP-1 sample extraction method by using a very reliable Peptide Extraction cartridge with excellent recovery and reproducibility. The procedure is easy to perform, and well-suited for managing a large number of samples without special equipment.