FluoBolt-WNT3A Fluorescence Immunoassay
The FluoBolt-WNT3A Fluorescence Immunoassay is manufactured in Austria by Fianostics GmbH
Method: Metal Enhanced Direct Sandwich Fluorescence Immunoassay
Size: 1×96 wells
Sensitivity: LOD (0 pmol/l + 3 SD): 51 pmol/l; LLOQ: 175 pmol/l
Dynamic Range: 0-2800 pmol/l
Incubation Time: Overnight
Sample Type: Serum, plasma
Sample Size: 20 µl
For Research Use Only
This platform is fully compatible to standard laboratory methodology using 96 well microtiter plate format and assays based on this technology can be run on any standard fluorescence microplate reader.
This FluoBolt WNT3A Fluorescence Immunoassay is regularly delivered standard with AlexaFluor680 labeled detection-antibody. If you wish to use a different dye, the following options are available:
- AlexaFluor680 (FIA-1705-A6)
- FITC labeled (FIA-1705-F)
- Cy3 labeled (FIA-1705-C3)
- Cy5 labeled (FIA-1705-C5)
Conversion factor: 1 ng/ml = 25 pmol/l (MW: 39.4 kDa)
Background
WNT3A is a secreted glycoprotein and belongs to the WNT family. Members of this family can interact with cell membrane receptors, thus playing a role in autocrine regulations and paracrine signaling. WNT3A is expressed in placenta at moderate levels, as well as in lung, spleen and prostate at low levels. The canonical sequence of WNT3A consists of 352 amino acids (aa) and has a mass of 39.365 kDa. It is rich in cysteine and forms many disulfide bonds from cysteine residues. At aa 87 and aa 298 glycosylation appears, since N-Acetylglucosamine is covalently attached to asparagine. At position aa 209 WNT3A is covalently lipidated at a conserved serine residue resulting in strong hydrophobic properties of the molecule. Therefore, in its physiological form it constitutes a soluble complex with afamin, which functions as a carrier for hydrophobic molecules in body fluids and is essential for the activity and solubility of WNT3A. WNT3A plays important roles in cell growth and differentiation, embryonic development, neural development, immune regulation, bone formation and carcinogenesis.
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