2-Naphthol ELISA Assay Kit


The 2-Naphthol ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of 2-Naphthol levels in biological samples. The Eagle Biosciences 2-Naphthol ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

SKU: NAP31-K01 Categories: , ,

2-Naphthol ELISA Assay Kit

The 2-Naphthol ELISA Assay Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: 10 pg/ml
Incubation Time: 2.5 hours
Sample Type: Serum, Plasma, cells, tissue, cell culture media, urine
Sample Size: 100 μL
Alternate Name: 2-NAP ELISA

This kit will obtain optimal results if all of the components are stored at the proper temperature prior to use. Items should be stored at the designated temperatures upon receipt of this kit. All components are stored below -20°C and should not be re-frozen and thawed more than necessary.

Assay Background

Burning fossil fuels and woods produce toxic PMs 2.5 and 10 mixed with polycyclic aromatic hydrocarbons (PAHs) which increase risk of cardiovascular diseases and lung cancer. Naphthalene is one of the major PAHs in the PMs (4.5%-13.5%). Inhaled PAHs through ambient air or occupationally exposed are biologically oxidized and excreted primarily by urine. Among various PAH metabolites, the level of urinary 2-naphthol was significantly correlated with PM 2.5 or PM 10 exposure, especially after wood smoke exposure. Burn pit smoke exposure may correlate to various health problems of soldiers.

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Additional Information

Assay Principle

Each kit for triplicate analyses of up to 24 samples contains one 96-well plate, one tube of 2-naphthol standard, one tube of 2-naphthol-conjugated HRP, and buffers for sample and HRP dilutions, and plate.
The 2-naphthol level in urine or cell culture media can be measured using the 2-naphthol ELISA, without ethyl acetate extraction after 4 to 10-fold dilution of the sample. This kit can be used for the determination of 2-naphthol in serum, plasma, cells, and tissues following proper isolation and purification.

Typical Standard Curve

2-Naphthol ELISA Assay Kit


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