Eye Epithelium expressing KillerRed Membrane

$4,025.00

The ARPE-19 Eye Epithelium expressing KillerRed in the membrane is for research use only and not use in diagnostic procedures.

SKU: ARPE-KillerRed-Membrane Categories: ,

Eye Epithelium expressing KillerRed Membrane

The Eye Epithelium expressing KillerRed Membrane is For Research Use Only

Human eye epithelium cells were stably transfected with KillerRed-Membrane to establish a cell line with bright red fluorescent cellular membranes, while no fluorescence is found in the cytoplasm. As a result, the plasma membrane with all its protrusions becomes clearly visible.

KillerRed is the first genetically encoded photosensitizer. It has been developed on the basis of the Anthomedusae chromoprotein anm2CP. Unlike chemical photosensitizers, KillerRed can be directly expressed by target cells, both individually and infusion with a target protein. It shows no cell toxicity before light activation. Upon green light irradiation, KillerRed generates reactive oxygen species (ROS) that damage the neighboring molecules. KillerRed phototoxicity is induced by green light irradiation at 540-580 nm and depends on light intensity, irradiation time and KillerRed concentration. (Description taken from Evrogen) This KillerRed-Membrane expressing cell line is a unique model to study light-induced killing of individual cells.


Specifications

Cell Line: ARPE-19 cells expressing red fluorescent KillerRed-Membrane

Cells/vial: approximately 5 x 105

Subculturing: 1:3 to 1:5

Medium Renewal: 2 to 3 times per week

Growth Medium: 1:1 Ham’s F12/ DMEM (3,1 g/L Glucose) + 10 % fetal calf serum (FCS), 4 mM L-Alanyl-LGlutamine, 2 mM Sodium Pyruvate, 1 % Non Essential Amino Acids (NEA)

Freeze Medium: Complete growth medium + 10% FCS, 10% DMSO, mycoplasma-free

Shipping: Frozen

Storage Recommendation: Liquid Nitrogen

Biosafety Level: 1


Related Products

Clone M-3 Mouse Melanoma expressing PhiYFP
Clone M-3 Mouse Melanoma expressing JRed
Ehrlich-Ascites-Tumor-mouse expressing Far-Red Turbo FP635

Additional Information

Package Inserts


Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

Product Citations