Mouse brain tumor cells expressing PhiYFP:
For Research Use Only
A bright yellow fluorescent cell line has been established by stable transfection with a plasmid coding for PhiYFP. The BC3H1 cell line was estalished in 1974 from a nitrosoethylurea-induced tumor. It displays many properties of skeletal muscle cells in two distinct morphologies: large spheroid cells and smooth-muscle-like cells. The BC3H1 cell line was described to express an acetylcholine-receptor and synthesize adenylate and creatine phosphokinases.
Cell Line: BC3H1 cells expressing PhiYFP
Cells/vial: approximately 5 x 105
Subculturing: 1:2 to 1:5
Medium Renewal: 2 to 3 times per week
Growth Medium: DMEM (4,5 g/L D-Glucose, 3,7 g/L NaHCO3) + 20 % fetal calf serum (FCS), 2 mM L-Alanyl-L-Glutamine, 2 mM Sodium Pyruvate, 1 % Non Essential Amino Acids (NEA)
Freeze Medium: Complete growth medium + 10% FCS, 10% DMSO
Storage Recommendation: Liquid Nitrogen
Biosafety Level: 1
This product contains a proprietary nucleic acid from coding for a proprietary fluorescent protein(s) intended to be used for research use only.