BC3H1 Mouse Myoblast expressing PhiYFP


SKU: BC3H1-PhiYP Categories: ,


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Mouse brain tumor cells expressing PhiYFP:
For Research Use Only

A bright yellow fluorescent cell line has been established by stable transfection with a plasmid coding for PhiYFP. The BC3H1 cell line was estalished in 1974 from a nitrosoethylurea-induced tumor. It displays many properties of skeletal muscle cells in two distinct morphologies: large spheroid cells and smooth-muscle-like cells. The BC3H1 cell line was described to express an acetylcholine-receptor and synthesize adenylate and creatine phosphokinases.


Cell Line:   BC3H1 cells expressing PhiYFP

Cells/vial:   approximately 5 x 105

Subculturing:    1:2 to 1:5

Medium Renewal:    2 to 3 times per week

Growth Medium:   DMEM (4,5 g/L D-Glucose, 3,7 g/L NaHCO3) + 20 % fetal calf serum (FCS), 2 mM L-Alanyl-L-Glutamine, 2 mM Sodium Pyruvate, 1 % Non Essential Amino Acids (NEA)

Freeze Medium:    Complete growth medium + 10% FCS, 10% DMSO

Shipping:    Frozen

Storage Recommendation:   Liquid Nitrogen

Biosafety Level: 1



This product contains a proprietary nucleic acid from coding for a proprietary fluorescent protein(s) intended to be used for research use only.