Equine Insulin ELISA Assay Kit
The Equine Insulin ELISA Assay Kit is For Research Use Only
Size: 1×96 wells
Sensitivity: 3µU/ml
Standard Range: 3µU/ml – 120µU/mL
Incubation Time: 1 hr 15 minutes
Sample Type: Serum and plasma
Sample Size: 25 µl
Assay Principle
The Equine Insulin assay is a two-site ELISA. The micro-plate is pre-coated with a monoclonal antibody against insulin. Standards and samples are added into the wells and co-incubated with a monoclonal antibody conjugated to horseradish peroxidase (HRP) enzyme. After wash step to remove any unbound substances, TMB substrate is added and color develops in proportion to the amount of insulin bound initially. The assay is stopped and the optical density of the wells determined using a micro-plate reader. Since the increases in absorbance are directly proportional to the amount of captured insulin, the unknown sample concentration can be interpolated from a reference curve included in each assay.
Sample Handling
No dilution of the sample is required in this assay, if a sample has an insulin level greater than the highest standard, the sample should be diluted with 0 µU/ml insulin standard solution and the assay should be repeated.
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