Degarelix (Firmagon) ELISA Assay Kit

$970.00

The Eagle Bioscience’s Degarelix (Firmagon) ELISA Assay Kit is used to estimate the level of Degarelix in human serum, plasma, and cell culture supernatant. The Degarelix (Firmagon) ELISA Assay Kit is intended for research use only and not to be used for diagnostic procedures.

SKU: KBI5008 Categories: , ,

Degarelix (Firmagon) ELISA Assay Kit

The Degarelix (Firmagon) ELISA Assay Kit Assay Kit is For Research Use Only

Size: 12 x 8 wells
Standard Range: 62.5-2000 ng/ml
Sensitivity: 40 ng/ml
Incubation Time: Minimum 2 hours and 15 minutes
Sample Type: Serum, plasma, and cell culture supernatant
Sample Size: 100 µL


Assay Principle

The method employs the quantitative sandwich enzyme immunoassay technique. The ELISA is a competitive immunoassay for the determination of Degarelix. A constant concentration of Degarelix coated on the microplate and varying concentration of standard or sample will compete for binding to gonadotropin-releasing hormone (GnRH) receptor. Detection antibody against GnRH conjugated to HRP is added to form a complex. The complex will produce a soluble colored product on substrate addition. The enzyme reaction is stopped by dispensing of Stop Solution into the wells. The optical density (OD) of the solution at 450 nm is inversely proportional to the amount of bound Degarelix present in standards or samples.


Sample Preparation and Storage

Serum – Use a serum separator tube and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 20 minutes at approximately 1,000×g. Assay freshly prepared serum immediately or store samples in aliquot at -20°C or -80°C for later use. Avoid repeated freeze/thaw cycles.

Plasma – Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 1,000×g at 2-8°C within 30 minutes of collection. Remove plasma and assay immediately or store samples in aliquot at -20°C or -80°C for later use. Avoid repeated freeze/thaw cycles.

Cell Culture Supernatants – Centrifuge samples for 20 minutes at 1,000×g. Collect the supernatant and assay immediately or store samples in aliquot at -20°C or -80°C for later use. Avoid repeated freeze/thaw cycles.


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Documents

Product Manual


Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

Additional Information

Background


Degarelix is used for the treatment of advanced prostate cancer. Degarelix is a synthetic peptide derivative drug which binds to gonadotropin-releasing hormone (GnRH) receptors in the pituitary gland and blocks interaction with GnRH. This antagonism reduces luteinizing hormone (LH) and follicle-stimulating hormone (FSH) which ultimately causes testosterone suppression. Reduction in testosterone is important in treating men with advanced prostate cancer. FDA approved the same as “Firmagon” in December 2008.

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