COVID-19 S-Protein Specific IgG Quantitative ELISA Assay Kit

$785.00

The COVID-19 S-Protein Specific IgG Quantitative ELISA Assay Kit is for the quantitative measurement of novel COVID-19 human IgG antibody to SARS-CoV-2 in human serum. This test is for research use only and not to be used for diagnostic or clinical procedures.

COVID-19 S-Protein Specific IgG Quantitative ELISA Assay Kit

COVID-19 S-Protein Specific IgG Quantitative ELISA Assay Kit was developed and manufactured in the US

Size: 1×96 wells
Sensitivity: 0.792 U/mL
Dynamic Range: 0-200 U/mL
Incubation Time
: 1 hr 20 mins
Sample Type: Serum
Species Sample: Human
Sample Size: 10 µL
Alternative Names: Coronavirus, COVID-19, SARS-CoV-2

For Research Use Only


Sample Collection and Storage

  • Only 10 μL of human serum is required for measurement in duplicate.
  • Samples should only be used on the same day.
  • Severe hemolytic samples should not be used.

Assay Principle

The COVID-19 S-Protein Specific IgG Quantitative ELISA Assay Kit is designed, developed, and produced for the quantitative measurement of novel COVID-19 human IgG antibody to SARS-CoV-2 in human serum. This assay utilizes the microplate based enzyme immunoassay technique.

Assay calibrators, controls, and 1:100 diluted human serum samples are added to the microtiter wells of a microplate that was coated with COVID-19 recombinant full length spike protein. After the first incubation period, the unbound protein matrix is removed with a subsequent washing step. A horseradish peroxidase (HRP) labeled polyclonal goat anti-human IgG tracer antibody is added to each well. After an incubation period, an immunocomplex of “COVID-19 recombinant antigen – human anti-COVID-19 IgG antibody – HRP labeled anti-human IgG tracer antibody” is formed if there is specific coronavirus IgG antibody present in the tested specimen. The unbound tracer antibody is removed by the subsequent washing step. HRP-labeled anti-hIgG tracer antibody bound to the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the tracer antibody bound to the anti-COVID-19 IgG on the wall of the microtiter well is proportional to the amount of the anti-COVID-19 IgG antibody level in the tested specimen.


COVID-19 S-Protein Specific IgG Quantitative ELISA Assay Kit

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Additional Information

Assay Background


2019 novel coronavirus (2019-nCoV or SARS-CoV-2 or COVID-19) is a single-stranded RNA coronavirus. Comparisons of the genetic sequences of this virus have shown similarities to SARS-CoV and bat coronaviruses. In humans, coronaviruses cause respiratory infections. Coronaviruses are composed of several proteins including the spike (S), envelope (E), membrane (M), and nucleocapsid (N). Results suggest that the spike protein retains sufficient affinity to the Angiotensin Converting Enzyme-2 (ACE-2) receptor to use it as a mechanism of cell entry. Human to human transmission of coronaviruses is primarily thought to occur among close contacts via respiratory droplets generated by sneezing and coughing. IgG is the most abundantly found immunoglobulin to be produced in response to an antigen and will be maintained in the body after initial exposure for long term response.

Documents

Product Manual


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