Total SARS-COV-2 IgG

COVID-19 Neutralizing Antibody Quantitative ELISA Assay Kit

$825.00

The COVID-19 Neutralizing Antibody Quantitative ELISA Assay Kit is an Enzyme-Linked Immunosorbent Assay (ELISA) kit intended for the quantitative measurement of the neutralizing antibody to SARS-CoV-2 receptor binding domain (RBD) of its spike protein in human serum. This kit is for research use only and not to be used for diagnostic or clinical procedures.

SKU: KTR-1036 Categories: , , ,

COVID-19 Neutralizing Antibody Quantitative ELISA Assay Kit

COVID-19 Neutralizing Antibody Quantitative ELISA Assay Kit was developed and manufactured in the US

Size: 1×96 wells
Dynamic Range: 0-100 U/mL
Incubation Time: 1.5 Hours
Sample Type: Serum
Species Sample: Human
Sample Size: 50 µL
Alternative Names: Coronavirus, COVID-19, SARS-CoV-2

For Research Use Only

Sample Collection and Storage

  • Only 10 μL of human serum is required for measurement in duplicate.
  • Samples should only be used on the same day.
  • Severe hemolytic samples should not be used.

Assay Principle

The COVID-19 Neutralizing Antibody Quantitative ELISA Assay Kit is designed, developed, and produced for the quantitative measurement of the neutralizing antibodies to SARS-CoV-2 RBD of its spike protein in human serum.

This assay utilizes the microplate-based enzyme immunoassay technique. Assay calibrators, controls, and human serum samples are added to the microtiter wells of a microplate coated with streptavidin. Simultaneously, horseradish peroxidase (HRP) labeled COVID-19 recombinant spike protein and biotinylated angiotensin converting enzyme-2 (ACE-2) are added to each well. After the first incubation period, the unbound protein matrix is removed with a subsequent washing step; a complex of “Streptavidin—Biotin-ACE2—HRP-COVID-19 recombinant spike protein” is formed. If there is specific COVID-19 neutralizing antibody present in the tested specimen, the formation of the above complex is blocked. A color reaction with a substrate solution in a timed reaction is measured in a spectrophotometric microplate reader. The HRP enzymatic activity of the complex on the wall of the microtiter well is inversely proportional to the amount of the COVID-19 neutralizing antibody level in the tested specimen.

COVID-19 Neutralizing Antibody Quantitative ELISA Assay Kit

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Anti-SARS-CoV-2 S1 (RBD) IgG ELISA Assay
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Additional Information

Assay Background

2019 novel coronavirus (2019-nCoV or SARS-CoV-2 or COVID-19) is a single-stranded RNA coronavirus. Comparisons of the genetic sequences of this virus have shown similarities to SARS-CoV and bat coronaviruses. In humans, coronaviruses cause respiratory infections. Coronaviruses are composed of several proteins including the spike (S), envelope (E), membrane (M), and nucleocapsid (N). Results suggest that the spike protein retains sufficient affinity to the Angiotensin Converting Enzyme-2 receptor to use it as a mechanism of cell entry. Human to human transmission of coronaviruses is primarily thought to occur among close contacts via respiratory droplets generated by sneezing and coughing. IgG is the most abundantly found immunoglobulin to be produced in response to an antigen and will be maintained in the body after initial exposure for long term response.

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