The Eagle Bioscience’s Bovine Betacellulin is for research use only and not to be used for diagnostic procedures.
Produced in E.coli using a patented expression system.
Bovine Betacellulin (BTC), a member of the EGF family, is an 80 amino acid heparin-binding protein. Like other members of the EGF family, it is synthesized as a transmembrane precursor. The soluble cytokine, containing one EGF structural motif, is released by proteolytic cleavage. BTC can bind to the EGF receptor (ErbB-1) which then dimerizes with another member of the ErbB receptor family to signal through the tyrosine kinase pathway. BTC is a potent mitogen for Balb/c 3T3 fibroblasts, retinal pigment epithelial cells and vascular smooth muscle cells and is the only member of the EGF family to stimulate insulin secretion in pancreatic beta cells.
- Purity: >95 % of the two BTC variants (by SDS-PAGE)
- Quantity: 100 µg
- Molecular Weight: Theoretical molecular weight is 8995 daltons
- N-terminal sequence analysis: Analysis of 5 residues demonstrating and equal mixture of [Gly1] bovine betacellulin (50%) and Des [1-4] bovine betacellulin
- Biological Activity: Stimulation of cell growth in Balb/c 3T3 fibroblasts (ED50 < 10 ng/ml)
- Endotoxin (LAL): <0.1 EU/ug
- Storage/Stability: At least 2 years at 2-4°C (lyophilized)
- Appearance: White powder freeze-dried from 0.05M acetic acid/0.05% (v/v) trifluoroacetic acid and stored under nitrogen at a slight vacuum.