Bioactive Sclerostin ELISA Assay Kit
Bioactive Sclerostin ELISA Assay Kit Developed and Manufactured in Austria by Biomedica
Size: 1×96 wells
Sensitivity: LOD: LOD: (0 pmol/l + 3 SD): 1.9 pmol/l; LLOQ: 1.3 pmol/l
Dynamic Range: 0 to 320 pmol/l
Incubation Time: > 4 hours
Sample Type: Serum, EDTA plasma, and citrate plasma (cell culture and urine protocol available)
Sample Size: 20 µL
Alternative Names: CDD, VBCH, DAND6, SOST1, SOST
For Research Use Only
Unit conversion: 1 pg/ml = 0.044 pmol/l (MW: 22.5 kD)
Specificity: Endogenous and recombinant bioactive Sclerostin.
Median serum (n=32): 61.5 pmol/l
Median EDTA plasma (n=24): 87 pmol/l
Median citrate plasma (n=24): 61.5 pmol/l
Each laboratory should establish its own reference range for the samples under investigation. Do not change sample type during the study.
The bioactive Sclerostin ELISA kit is a sandwich enzyme immunoassay for the quantitative determination of bioactive Sclerostin (SOST) in human serum and plasma samples. In a first step, assay buffer is pipetted into the wells of the microtiter strips. Thereafter, standard/control/sample are pipetted into the wells, which are pre-coated with a recombinant monoclonal anti-human Sclerostin antibody. Any bioactive Sclerostin present in the standard/control/sample binds to the pre-coated antibody in the well. After incubation, a washing step is applied where all non-specific unbound material is removed. In a next step, the conjugate (anti-human Sclerostin-HRP) is pipetted into the wells and reacts with bioactive Sclerostin present in the sample, forming a sandwich. After another washing step, the substrate (tetramethylbenzidine; TMB) is pipetted into the wells. The enzyme-catalyzed color reaction of the substrate is directly proportional to the amount of bioactive sclerostin in the sample. This color change is detectable with a standard microtiter plate reader. A dose response curve of the absorbance (optical density, OD, at 450 nm) versus standard concentration is generated, using the values obtained from the standards. The concentration of bioactive Sclerostin in the sample is determined directly from the dose response curve.