Eosinophil Derived Neurotoxin ELISA Assay
The Eosinophil Derived Neurotoxin ELISA Assay is For Research Use Only
Size: 1×96 wells
Sensitivity: 0.164 ng/mL
Incubation Time: 2.5 hours
Sample Type: Stool, Urine, Serum, Plasma
Sample Size: 100 µl
Stool (n= 87): preliminary cut off 360 ng/ml
In a study the EDN concentration was measured in 87 probands and the preliminary cut off value was found.
Urine (n=50): 81.8 (26.7 – 164.2) µg/mmol creatinine
Serum (n=52): 26,4 (8,3 – 66,4) ng/ml
Plasma (n=52): 18,1 (6,2 – 49,8) ng/ml
We recommend that each laboratory develop their own normal range. The values mentioned above are only for orientation and can deviate from other published data.
After activation eosinophil granulocytes segregate the cationic glycoprotein EDN (eosinophil derived neurotoxin). This 18-21 kDa single stranded glycosylated protein is also known as EPX (eosinophil protein X). Together with ECP (eosinophil cationic protein), EDN belongs to the ribonuclease superfamily1-3. EDN, however, has a 100-fold increased ribonuclease activity. EDN is also neurotoxic and not cytotoxic4,5. The activation of eosinophil granulocytes is important during the inflammatory processes in allergic reactions. Thus EDN is a marker for eosinophil activation and degranulation.
The measurement of EDN in stool allows the detection of clinical or subclinical chronic inflammation in the gut. Some research has suggested that the measurement of EDN gives information on the activity of disease and the prediction of a relapse. The Eosinophil Derived Neurotoxin (EDN) ELISA Assay Kit allows an easy, rapid and precise quantitative determination of the eosinophil derived neurotoxin in biological samples. The kit includes all reagents ready to use for preparation of the samples.