Anti-Mullerian Hormone ELISA Assay
Anti-Mullerian Hormone ELISA Assay developed and manufactured in the USA
Size: 1×96 wells
Sensitivity: 0.02 ng/mL
Dynamic Range: 0.11-20 ng/mL
Incubation Time: 4.5 hours
Sample Type: Serum, EDTA Plasma and/or Lithium Heparin Plasma
Sample Size: 50 µL
Alternative Names: AMH ELISA Assay Kit
For Research Use Only
Assay Principle for Anti-Mullerian ELISA
The Anti-Müllerian Hormone (AMH) ELISA is designed, developed and produced for the quantitative measurement of human AMH in serum or heparin plasma samples. The AMH assay utilizes the two-site “sandwich” technique with two selected antibodies that bind to different epitopes of human AMH.
In this AMH Assay, calibrators, controls and patient samples are added directly to wells of a microtiter plate that is coated with antibody to N-terminal AMH along with another AMH specific antibody labeled with horseradish peroxidase (HRP). After an initial incubation period, the plate is washed a sandwich of solid-phase antibody – human AMH – HRP-conjugated monoclonal antibody is formed. The unbound monoclonal antibodies and buffer matrix are removed in the subsequent washing step. For the detection of this immunocomplex, the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to the wall of each microtiter well is directly proportional to the amount of human calprotectin in the test sample. A standard curve is generated by plotting the absorbance verse the respective human calprotectin concentration for each standard on a Cubic or point-to-point curve fitting. The concentration of human AMH in test samples is determined directly from this calibration curve.