Human CD178 ELISA Assay
The Human CD178 ELISA Assay is For Research Use Only
Size: 1×96 wells
Sensitivity: 12 ng/ml
Dynamic Range: 62.5 – 2000 pg/ml
Incubation Time: 3h 45 min
Sample Type: Serum, Plasma, Cell Culture
Sample Size: 100 µl
Alternative Names: Fas-L, Fas Ligand, CD95-L
The Human CD178/ Fas-L ELISA Assay kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for Fas-L has been coated onto the wells of the microtiter strips provided. Samples, including standards of known Fas-L concentrations and unknowns are pipetted into these wells.
During the first incubation, the Fas-L antigen is added to wells. After washing, a biotinylated monoclonal antibody specific for Fas-L is incubated. Then the enzyme ( streptavidin-peroxydase ) is added. After incubation and washing to remove all unbound enzyme, a substrate solution which acts on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Fas-L present in the samples.
Store the Human CD178/ Fas-L ELISA Assay kit reagents between 2 and 8°C. Immediately after use remaining reagents should be returned to cold storage (2-8°C). Expiry of the kit and reagents is stated on box front labels. The expiry of the kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, the reagent is not contaminated by the first handling.