Anti CP IgG ELISA Assay Kit

$380.00

The Eagle Biosciences Anti CP IgG ELISA Assay Kit is an indirect solid phase enzyme immunometric assay (ELISA) kit designed for the quantitative measurement of IgG class antibodies directed against Citrullinated Peptides (CP) in human serum or plasma. Anti CP IgG ELISA Assay Kit is intended for research use only and not to be used in diagnostic procedures.

SKU: DCM149 Categories: , ,

Anti CP IgG ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 5 AU/ml
Dynamic Range: 5 – 80 AU/ml
Incubation Time: 1.5 hour
Sample Type: Serum, Plasma
Sample Size: 10 µL

Controls Included

Product Developed and Manufactured in Italy by Diametra

Additional Information

Assay Background

Rheumathoid Arthritis (RA) is one of the most common autoimmune diseases (1-2% European population). The most significant clinical symptom is an inflammation of the synovial membranes which causes a painful swelling of the articulations and the ankylosis.  In order to correctly diagnose RA it is necessary to exclude other forms of arthritis: in this diagnostic process, the laboratory plays an important role in the determination of Rheumatoid Factor (RF) antibodies of class IgM, detectable in 60-80% of the patients with RA. The RF antibodies are sensitive but not very specific markers.  On the contrary, anti citrullinated peptides (CP) autoantibodies are a hallmark of RA and are used in diagnostic assays, through the use of citrullinated sequences (such as: filaggrin and filaggrin derived cyclized peptides, citrullinated recombinant vimentin, fibrin, viral citrullinated proteins) as antigens.  Eagle Biosciences’ Anti CP IgG ELISA Assay Kit is based on viral and human citrullinated peptidic sequences that are used as antigens; this combined use allows to detect different subgroups of anti CP antibodies in the same set of patients, leading to a better diagnostic investigation.

Assay Principle

The Anti CP IgG ELISA Assay Kit is based on the binding of antibodies present in calibrators, controls or prediluted patient samples to the syntetic Citrullinated Peptides (CP) coated on the inner surface of the wells. After a 30 minutes incubation the microplate is washed with a wash buffer to remove the non-reactive serum components.  Then an anti-human-IgG horseradish peroxidase conjugated solution recognizes the IgG class antibodies bound to the immobilized antigens. After a 30 minutes incubation, any excess of enzyme conjugate not specifically bound is washed away with the wash buffer.  Finally a chromogenic substrate solution containing TMB is dispensed into the wells. After 15 minutes of incubation the color development is stopped by adding the stop solution. The solutions color changes into yellow. The amount of color is directly proportional to the concentration of IgG antibodies present in the original sample.

Manual

Product Manual