Progesterone Saliva ELISA Assay
The Progesterone Saliva ELISA Assay is For Research Use Only
Size: 1×96 wells
Sensitivity: 20 pg/mL
Dynamic Range: 20–5000 pg/mL
Incubation Time: 80 minutes
Sample Type: Saliva
Sample Size: 50 µL
The principle of the Progesterone Saliva enzyme immunoassay test follows the typical competitive binding scenario. Competition occurs between an unlabelled antigen (present in standards, controls and patient samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microplate. The washing and decanting procedures remove unbound materials. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed is inversely proportional to the concentration of progesterone in the sample. A set of standards is used to plot a standard curve from which the amount of progesterone in patient samples and controls can be directly read.
SPECIMEN COLLECTION AND STORAGE
Approximately 1 mL of saliva is required per duplicate determination. Collect 4–5 mL of saliva into a clean glass tube* between 7–10 am without force or inducement and before eating, drinking or brushing the teeth. Simply rinse the mouth with water before collection. Do not use blood-contaminated specimens. Store samples at 4°C for up to 24 hours or at -10°C or lower if the analyses are to be done at a later date. Consider all human specimens as possible biohazardous materials and take appropriate precautions when handling.
*Do not use cotton or polyester rolls or plastic collection tubes for collecting saliva samples in this assay, since it has been well established that false elevated results will occur.