Active Ghrelin ELISA Assay Kit
The Active Ghrelin ELISA Assay Kit is For Research Use Only
Size: 1×96 wells
Dynamic Range: 2.9 – 184 fmol/mL
Incubation Time: 3 hours 30 minutes
Species: Human, Rat, Mouse
Sample Type: Plasma
Sample Size: 50 µL
Alternative Name: Acylated Ghrelin
Assay Principle
This kit measures the active form of Ghrelin based on the principle of 2 Site Sandwich enzyme-linked immunosorbent assay (ELISA). It can detect not only octanoylated human Ghrelin but also octanoylated rat/mouse Ghrelin (1-28). This kit is manufactured using the high specific antibody pairs generated by Dr. Kangawa and by following his protocol.
Assay Background
Ghrelin, a novel growth hormone releasing peptide is an acylated peptide that stimulates the release of growth hormone from pituitary. It was isolated from rat stomach and the structure was determined as a peptide consisting of 28-amino acid by Dr. Kenji kangawa (National Cardiovascular Center in Japan). The Ser3 residue of Ghrelin is modified by n-octanoic acid, a modification necessary for hormone activity.
Preparation of Sample
Ghrelin is very unstable. Be careful to avoid any fragmentation of inactivation. All biological fluid should be treated with protease inhibitor such as aprotinin. It is also required to inhibit the esterase activity. Standard procedure of human blood sample preparation is as described below.
Collect into the bleeding tubes which contain 500 KIU (Kallikrein Inhibitor Unit) of aprotinin and 1.25 mg of EDTA-2Na per 1 mL of whole blood. Rock the tubes gently and then immediately centrifuge the blood sample (1500xg, 15 min at 4°C). Plasma should be immediately treated with 1/10 volume of 1 mol/L HCl. Sample must be kept below -40°C for long term storage.
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