8-OHdG Ultrasensitive ELISA Assay
The 8-OHdG Ultrasensitive ELISA Assay is For Research Use Only
Size: 1×96 wells
Sensitivity: 0.125 ng/mL
Dynamic Range: 0.125 – 10 ng/mL
Incubation Time: Overnight
Sample Type: Serum, Urine, Biological Fluids
Sample Size: 50 µL
Alternative Names: 8-hydroxy-2’-deoxy-guanosine
Product manufactured in the USA
Bring all reagents of the to room temperature before beginning 8-hydroxy-2′-deoxy-guanosine (8-OHdG) Ultrasensitive ELISA assay kit. Determine the number of microwells needed for the assay (each sample, standard, and control should be assayed in duplicate). Bring all reagents and samples to room temperature (20-25ºC) before use.
The 8-OHdG monoclonal antibody and the sample or standard are added to the microtiter plate which has been precoated with 8-OHdG. The 8-OHdG monoclonal antibody reacts competitively with the 8-OHdG bound on the plate and the 8-OHdG in samples solution. Therefore higher concentrations of 8-OHdG in the sample solution lead to a reduced binding of the antibody to the 8-OHdG on the plate.
The antibodies which are bound to the 8-OHdG in the sample are washed away from the antibodies that have bound to the 8-OHdG coated on the plate.
An enzyme-labeled secondary antibody, which is added to the plate, binds to the monoclonal antibody which is bound to the 8-OHdG coated on the plate.
Unbound enzyme-labeled secondary antibody is removed by a wash step.
Addition of a chromatic substrate results in the development of color in proportion to the amount of antibody bound to the plate.
The color reaction is terminated and the absorbance is measured.