8-hydroxy-2-deoxyguanosine ELISA Assay Kit
The 8-hydroxy-2-deoxyguanosine ELISA Assay Kit is For Research Use Only
Size: 1×96 wells
Sensitivity: 0.5 ng/mL
Dynamic Range: 0.5 – 200 ng/mL
Incubation Time: 3.5 hours
Sample Type: Serum, Urine, Biological Fluids
Sample Size: 50 µL
Alternative Names: 8-OHdG
Bring all reagents to room temperature before beginning 8-hydroxy-2′-deoxy-guanosine (8-OHdG) ELISA assay kit. Determine the number of microwells needed for the assay (each sample, standard, and control should be assayed in duplicate). Bring all reagents and samples to room temperature (20-25ºC) before use.
The anti-8-OHdG monoclonal antibody and the sample or standard are added to the microtiter plate which has been precoated with 8-OHdG. The 8-OHdG monoclonal antibody reacts competitively with the 8-OHdG bound on the plate and the 8-OHdG in samples solution. Therefore higher concentrations of 8-OHdG in the sample solution lead to a reduced binding of the antibody to the 8-OHdG on the plate.
The antibodies which are bound to the 8-OHdG in the sample are washed away from the antibodies that have bound to the 8-OHdG coated on the plate.
An enzyme-labeled secondary antibody, which is added to the plate, binds to the monoclonal antibody which is bound to the 8- OHdG coated on the plate.
Unbound HRP-conjugated secondary antibody is removed by washing.
Addition of the substrate solution results in the development of color in proportion to the amount of anti 8-OHdG antibody bound to the plate.
The reaction is terminated by phosphoric acid, and absorbance at 450 nm is measured.