14,15 DHET ELISA Assay Kit
The 14,15 DHET ELISA Assay Kit is For Research Use Only
Product manufactured in the USA
Size: 1×96 wells
Sensitivity: 0.01 ng/ml
Dynamic Range: 0.01 – 1000 ng/ml
Incubation Time: 2.5 hours
Sample Type: Serum, Plasma, Tissue, Biological Fluids
Sample Size: 100 µl
Assay Background
This competitive 14,15 DHET ELISA Assay kit is for determination of 14,15-DHET levels in biological samples. Specificity of the 14,15 DHET ELISA has been published. The 14,15-DHET level exhibited strong positive correlation with hypertension, brain injury and stroke in rodents. 14,15-DHET is a representative metabolite of soluble epoxide hydrolase-mediated metabolism of EETs, which are generated by arachidonic acid epoxygenase activity of cytochromes P450. Human blood 14,15-DHET levels were measured using the 14,15-DHET ELISA kit. Increased 14,15-DHET levels of human cells as detected by the 14,15-DHET ELISA were indicative of the neoplastic and metastatic phenotype of carcinoma cells. This 14,15 DHET ELISA Assay kit can be used for the determination of 14,15-DHET in serum, plasma, cells, and tissues following proper isolation and purification.
This competitive ELISA kit, based on competition between 14,15-DHET epitope and 14,15-DHET-HRP conjugate for a limited number of binding sites available from the anti-14,15-DHET antibody, which is coated to the wells of the 96 well ELISA plate. The conjugate concentration is held as a constant in each well, while the concentration of the 14,15-DHET is variable, based on the concentration of the sample or standard. Thus the amount of the 14,15-DHET conjugate which is able to bind to each of the wells is inversely proportional to the concentration of 14,15-DHET in the standard or sample. The amount of the conjugate which is bound to each well is then determined by the amount of color obtained when TMB is added. The TMB reacts with the HRP available in the well. With the addition of sulfuric acid, the blue colored product is converted into a yellow colored product, which can be read on a plate reader at 450 nm.
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