What Is It?
This Eagle Biosciences High Sensitivity Glucagon ELISA Assay Kit is used for quantitative determination of glucagon in plasma, serum and culture medium supernatant. The kit is characterized by its sensitive quantification and high specificity. In addition, it has no influence by other components in samples.
How Does It Work?
This ELISA kit for determination of glucagon is based on a sandwich enzyme immunoassay with two monoclonal antibodies. Standards or samples, and HRP labeled antibodies are added to the wells of plate coated with antibodies against glucagon. During the incubation antibody – antigen – labeled antibody complex is formed on the surface of the wells. After the incubation and rinsing out excess labeled antibody, HRP enzyme activity is finally determined by 3,3’,5,5’-Tetramethylbenzidine (TMB) and the concentration of glucagon is calculated.
What Is It’s Specifity?
This Eagle Biosciences High Sensitivity Glucagon ELISA Assay Kit has high specificity to glucagon, and shows no significant cross reactivity to Glicentin, Oxyntomodulin, GLP-1 and GLP-2.
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