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EagleBIo’s Intact FGF-21 ELISA Cited in Recent Boston Study

by Eagle Biosciences

EagleBio’s Intact FGF-21 ELISA
was recently utilized in Boston study by Beth Israel Deaconess Medical Center,
Harvard Medical School. This assay is the first Intact FGF-21 ELISA
assay kit that truly measures the active human intact FGF-21 (1-181) with no
cross reaction to any FGF-21 fragments.

Shenoy, V. et. al. Elevated Serum
Fibroblast Growth Factor 21 in Humans with Acute Pancreatitis. PLOS One. 2016. https://dx.doi.org/10.1371/journal.pone.0164351

Highlight from Publication:

“To assess the possibility that FGF21 is
released from the pancreas during acute injury in humans, we measured
circulating FGF21 in subjects with acute pancreatitis over the course of their
disease. We observed that acute pancreatitis subjects had significantly
increased peak circulating FGF21 values compared to healthy controls. Further,
peak FGF21 levels were observed four to six days after lipase levels peaked.
The timing of this elevation suggests that FGF21 may play a role in or be a
marker of disease recovery.” Read More

Source: (https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0164351
)

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Trifluoperazine Inhibits Disordered Protein In Pancreatic Tumors

by Eagle Biosciences


A recent research study focused on screening more than 1,000 drugs already approved for various therapeutic indications. The objective was to find which drugs are capable of inhibiting the protein involved in the pancreatic cancer development process, Nupr1.

“Trifluoperazine, a molecule long used to treat anxiety has been found to interfere with the activity of a protein involved in the pancreatic cancer development process. A study by the Nanotechnology Institute of CNR, unit of Rende, in collaboration with a team of French and Spanish researchers, identified the molecule.

Pancreatic cancer is one of the most lethal tumors. Up until now, the only drugs available to fight it are generic chemotherapy treatments.”  Read More

Source: (https://reliawire.com/trifluoperazine-pancreatic-tumors/)

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EagleBio’s 25 OH Vitamin D ELISA Cited in University of Florida Study

by Eagle Biosciences


A recent
study was conducted by the University of Florida, Gainesville using
Eagle Bioscience’s 25-OH Vitamin D
ELISA from our Vitamin Assay Kit Line.
The Vitamin Assay
Kit line offered Eagle Biosciences provides unique and sensitive
assays for measuring a variety of vitamin analytes in numerous fields of
research. Check the product page for full details on our 25-OH Vitamin D
ELISA or view the links below for information about the study
referencing this kit.

Publication: 

Nelson, C. et al.  Vitamin D status of dairy cattle: Outcomes of current practices in the dairy industry. Journal of Dairy Science. December 2016, Volume 99 :12: 10150–10160. DOI: https://dx.doi.org/10.3168/jds.2016-117

“The need for vitamin D supplementation of dairy cattle has been known for the better part of the last century and is well appreciated by dairy producers and nutritionists. Whether current recommendations and practices for supplemental vitamin D are meeting the needs of dairy cattle, however, is not well known. The vitamin D status of animals is reliably indicated by the concentration of the 25-hydroxyvitamin D [25(OH)D] metabolite in serum or plasma, with a concentration of 30 ng/mL proposed as a lower threshold for sufficiency. The objective of this study was to determine the typical serum 25(OH)D concentrations of dairy cattle across various dairy operations….” Read More 

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Product Highlights: ILite® Anti-CD20 ADCC Activity Set

by Eagle Biosciences

ILite® Anti-CD20 ADCC Activity Set is for Research Use Only in the US.  Not to be used for Diagnostic Procedures.

Eagle Biosciences is proud to announce the launch of the iLite ADCC Activity Assay product line and the iLite anti-CD20 ADCC Activity Set.

Rituximab, a chimeric monoclonal antibody against CD20, was one of the first therapeutic antibodies within the oncology field to receive approval by the FDA in 1997. Ever since biosimilar and innovative biologicals with similar mechanism of action have been developed and marketed.

Antibody-dependent cell-medicated cytotoxicity (ADCC) can now, using our new iLite®ADCC Activity Assays, be measured in a straight forward, sensitive and reproducible manner.

ILite® Anti-CD20 ADCC Activity Set is a great addition to EagleBio’s Drug Monitoring kit Line which offers a unique grouping of products that provide incredible value to pre-clinical and clinical pharmacology studies, cancer research, and more specifically anti-tumor therapeutic investigation and more.

Key Advantages

The iLite® anti-CD20 ADCC Activity Set

  • Comprehensive kit– Contains 3 different genetically modified cell lines (components also available separately):
    1. Reporter Effector cell,
    2. Positive Target cell
    3. Negative Target cell
  • Fast- results in 5 hours- this set of cells enables measurements of an anti-CD20 drug’s ability to induce ADCC,
  • Easy- NO Cell culturing isrequired
  • Flexible– Effector cells, which can be used with any Target cell, include a normalization readout which can adjust for differences in cell counts.
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EagleBio’s ACTH ELISA Used in Canadian Study

by Eagle Biosciences

 

ACTH ELISA was recently utilized in a Canadian Study. ACTH ELISA is part of
our Steroid Assay Kit Line ,
a product linewhich offers an array of interesting and sensitive
assays for different hormone analytes and research needs. Check the product
page for full details on the kit or view the links below to find a new
publication referencing our ACTH ELISA.

F.O.
Owagboriaye, et. al. Assessment of the effect of gasoline fume on stress
hormones, antioxidant status and lipid peroxidation in albino rat, Journal of
King Saud University – Science, Available online 2 December 2016, ISSN
1018-3647, https://dx.doi.org/10.1016/j.jksus.2016.11.002.

Publication Highlights:

“Gasoline fume has been considered a major air
pollutant affecting the heart, lungs, brain, liver and kidneys. Therefore, this
study aims at investigating the effect of inhalation exposure to gasoline fume
on some endogenic stress hormones and oxidative enzymes of albino rats……The
levels of adrenocorticotropic hormone (ACTH), aldosterone and corticosterone
were determined using enzyme-linked immunosorbent assay (ELISA) kits.” 

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EagleBio’s Functional Leptin ELISA Utilized in Recent Germany Study

by Eagle Biosciences

Martin Wabitsch, Lutz P”ridzun, Michael Ranke, Julia von Schnurbein, Anja Moss, Stephanie Brandt, Katja Kohlsdorf, Barbara Moepps, Michael Schaab, Jan-Bernd Funcke, Peter Gierschik, Pamela Fischer-Posovszky, Bertram Flehmig and Jürgen Kratzsch. Measurement of immunofunctional leptin to detect and monitor patients with functional leptin deficiency.European Journal of Endocrinology. 173:3, 315-322. DOI: 10.1530/EJE-16-0821.

The authors from German research centers Ulm, Leipzig and Tübingen, as well as scientists at Mediagnost report a new assay that measures Leptin binding capability to the leptin receptor. It therefore identifies individuals with bioinactive leptin due to mutations affecting receptor binding. It is thus an improvement over total immunoreactive leptin measurements to identify those with functional leptin insufficiency.

Highlight from Publication:

“The new Mediagnost bioLep assay L07 is able to detect impaired leptin bioactivity in severely obese patients with a homozygous gene defect as well as in heterozygous carriers of such mutations and is a time- and cost-effective diagnostic tool to identify these individuals and ultimately allow proper therapy to be initiated where applicable as early in life as possible.”

Functional Leptin ELISA

Functional Leptin ELISAis part our Endocrine Assay Kit Line, a line that offers a comprehensive product grouping of unique and for measuring a variety of analytes related to diabetes and obesity research.

This assay is:

  • Based on immobilized soluble leptin receptor and a highly specific polyclonal antibody: allowing researchers to measure biologically active / receptor binding leptin only
  • Sensitive, specific, and provides results within 4 hours
Categories

Product Spotlight: TK210 (Thymidine Kinase 1) ELISA Assay

by Eagle Biosciences

 

TK
210 ELISA, manufactured by AroCell is offered in EagleBio’s Cancer Biomarker Assay Line. The Cancer
Biomarker Assay Kit Line offered Eagle Biosciences provides a comprehensive
array of sensitive and valuable tools to assist researchers in new emerging
market segments in cancer research such as tumor marker and anti-tumor therapy
investigation.

Our TK
210 ELISA is a convenient, simple test as well as the most sensitive
ELISA for measuring TK1.

Why Measure Thymidine Kinase 1 (TK1)?

The enzyme Thymidine Kinase 1 (TK1) is up-regulated in
tumor cells from which it is released into the blood during their turnover.
Increased serum TK1 protein has been discovered to be associated with tumor
growth. TK1 up-regulation may be an early event in cancer development and TK1
may even be elevated in pre-cancerous conditions.1

Although TK1 activity is a valuable biomarker it has
limitations in that most current methods are based on enzyme activity
assays.The AroCell TK 210 ELISA test, based on two monoclonal antibodies
specific for a unique TK1 amino acid sequence (194-225), brings the
specificity, sensitivity and robustness of immunoassay to the assay of serum
TK1.2

Key Advantages of TK 210 ELISA:

  • Most
    Sensitive ELISA for measuring TK1
  • Simple
    ELISA procedure with same day results
  • Accurately
    measure both Active & Inactive TK1
  • Solid
    tumors do not affect results unlike other methods for measuring TK1
  • Strong
    Evidence that TK210 can be used successfully with traditional cancer
    markers like CA15-3
  • Use
    with both Serum or Tissue samples

Recent Citations:

View
Publications List

 

References:

1.Alegre,
M.M. et al. (2012). Thymidine Kinase 1 Upregulation Is an Early Event in Breast
Tumor Formation. Journal of Oncology. Volume 2012, 5 pages.

2.Kumar.
J.K. et al. (2016). A Clinical Evaluation of the TK 210 ELISA in Sera from
Breast Cancer Patients Demonstrates High Sensitivity and Specificity at All
Stages of the Disease. Tumor Biology. DOI 10.1007/s13277-016-5024-z (Epub ahead
of print)

Categories

Product Highlights: Chromogranin A ELISA

by Eagle Biosciences

Chromogranin A ELISA

Chromogranin A ELISA is for Research Use Only.

Eagle Biosciences’ Chromogranin A ELISA in our Cancer Biomarker Assay Kit Line. The Cancer Biomarker Assay Kit Line offered Eagle Biosciences provides a comprehensive array of sensitive and valuable tools to assist researchers in new emerging market segments in cancer research such as tumor marker and antitumor therapy investigation.

Our Chromogranin A ELISA is one of the unique assays in the this Cancer Biomarker Assay Kit Line that offers excellent  sensitivity, precise results in under 3 hours, and exclusive measurement of human chromogranin A without the high dose “hook” effect up to 1,000,000 ng/ml.

Why Measure Chromogranin A?

Chromogranin A (CgA) is a 49 kDa acidic protein that consists of 439 amino acids encoded on chromosome 14. It has been identified in a number of normal and neoplastic endocrine tissues. In fact, chromogranin A is a highly sensitive and specific marker for pheochromocytoma, carcinoids, and other neuroendocrine tumors (NET) [1]. Molina et. al describes “CgA is one of the most used tumor markers in NETs, and elevated CgA concentrations have been demonstrated in serum or plasma of patients with different types of these tumors [2].”

The measurement of Chromogranin A  provides incredible value to scientists studying numerous areas of cancer research beyond other biomarkers utilized for the detection, evaluation, and monitoring of specific tumor types.

Key Advantages of EagleBio’s Chromogranin ELISA, CHR31-K01

  • Excellent Sensitivity
  • Precise results under 3 hours
  • Measures CgA without the high dose “hook” effect up to 1,000,000 ng/ml.
  • Competitive Price

Recent Citations for EagleBio’s Chromogranin A ELISA

Conteduca et. al. Chromogranin A is a Potential Prognostic Marker in Prostate Cancer Patients Treated With Enzalutamide. Prostate. 2014 Dec;74(16):1691-6. PMID: 25262981.

Molina et al. Evaluation of chromogranin A determined by three different procedures in patients with benign diseases, neuroendocrine tumors and other malignancies. Tumor Biol. (2011) 32:13–2. PMID:20730520

Burgio SL.et. al. Chromogranin A predicts outcome in
prostate cancer patients treated
with abiraterone. Endocr Relat Cancer. 2014 May 8;21(3):487-93.  PMID: 24741024

 

References:

1. Pirker RA et. al, Usefulness of chromogranin A as a marker for detection of relapses of carcinoid tumours. Clin Chem Lab Med. 1998 Nov;36(11):837-40. PMID: 9877088

2. Molina et al. Evaluation of chromogranin A determined by three different procedures in patients with benign diseases, neuroendocrine tumors and other malignancies. Tumor Biol. (2011) 32:13–2. PMID:20730520

 

Categories

Unique, Early Biomarker for Cell Proliferation

by Eagle Biosciences

Introducing the TK 210 ELISA assay kit from AroCell, the most sensitive assay available for measuring Thymadine Kinase 1 (TK1). TK1 is a key enzyme in DNA replication and it is up-regulated in proliferating cells. Many studies have shown it to be a valuable cancer biomarker but most previous assays have been activity based and underestimate TK1 in solid tumors.&nbspRead More

 

TK 210 ELISA is for Research Use Only. Not for Use in Diagnostic Procedures in the US.

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EagleBio’s Serotonin ELISA Cited in a Michigan State University Study

by Eagle Biosciences

EagleBio’s Serotonin ELISA was used in a Michigan State Univeristy study (see citation below).  This is a kit from our Neurobiology Assay Kit line. The Neurobiology Assay Kit line offered Eagle Biosciences provides a comprehensive array of sensitive tools to measure catecholamines and numerous neuroscience-related analytes on various types of samples.

Plasma serotonin levels were determined in this study using Eagle Biosciences’ Serotoinin ELISA:

Joshi, N. Inhibition of PAR-4 and P2Y12 receptor-mediated platelet activation produces distinct hepatic pathologies in experimental xenobiotic-induced cholestatic liver disease. Toxicology. 2016; 365:9-16.