Author: Eagle Biosciences

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Cortisol measurement by EagleBio

by Eagle Biosciences
Cortisol measurement by EagleBio

Cortisol Saliva ELISA is for Research Use Only. Not for diagnostic procedures.

Cortisol measurement by EagleBio Cortisol Saliva ELISA compared to LC-MS/MS 

Salivary cortisol has been found to be a good index of stress with increased levels of cortisol correlating positively with increased levels of
stress. Cortisol levels rise slowly over time and take a while to return to base level, indicating that cortisol is more
associated with chronic stress levels. 

Diurnal variation is a major factor for within-person variance because baseline cortisol levels have been known to
differ based on the time of day. For normally developing individuals who follow a typical day–night schedule,cortisol
production peaks during the last few hours of sleep. This peak is thought to aid in preparing the body for action and
stimulate the appetite upon waking.Diurnal variation is also affected by psychological conditions.

For several years the gold reference standard for ELISA has been the RIA method.

Now Eagle Biosciences performed a new study with the support of Università Cattolica of Rome, comparing the ELISA
results with the LC-MS/MS technique (mass spectrometry), obtaining a very good correlation between the two
methods: R= 0.94. 

This study has been performed on 63 samples from healthy subjects, collected in different time. 

 

The Cortisol Saliva assay is now considered by scientific community a reliable alternative to Urinary Cortisol assay.

 

Why Use EagleBio’s Cortisol Saliva ELISA?

  • Easy stress-free, non-invasive method of salvia collection:

Making saliva one of the most accessible body fluids and potentially one of the most valuable in studying normal human physiology as well as pathology.

  • Excellent correlation between serum and plasma levels:

The majority of cortisol in saliva is not-bound and enters the saliva via intracellular mechanisms. Salivary cortisol levels are unaffected by salivary flow rate or salivary enzymes.

  • Excellent Correlation to LC-MS/MS

R= 0.94

 

Related Kits:

Steroid Assay Kits

Cortisol ELISA Assay Kit

Cortisol Saliva ELISA Assay Kit

Testosterone ELISA Assay Kit

Testosterone Saliva ELISA Assay Kit

 

Related News:

EagleBio’s Ultrasensitive Cortisol Saliva ELISA is a Valuable Tool in a Circadian Rhythmicity Study

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THC Destroys Amyloid Beta in Alzheimer’s (2016 Research)

by Eagle Biosciences
THC Destroys Amyloid Beta in Alzheimer’s (2016 Research)


Brain inflammation is also commonplace with Alzheimer’s disease. In June 2016’s Aging and Mechanisms of Disease, members of the Salk Institute team studied nerve cells altered to produce higher levels of the amyloid beta protein in order to mimic the conditions found in Alzheimer’s. High levels of the protein are associated with cellular inflammation and higher rates of neuronal death. Previously, it was thought that the inflammation occurred from immune cells, but an uncanny discovery proved otherwise—the amyloid beta proteins instead are the cause of inflammatory effects within the brain. Read More

Source:(https://agscientific.com)

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Recent Boston Study Utilizes EagleBio’s Intact FGF-21 ELISA Kit

by Eagle Biosciences


EagleBio’s Intact FGF-21 was recently utilized in this Boston Study at Tufts University:

Human FGF-21 Is a Substrate of Fibroblast Activation Protein

FGF-21 is a key regulator of metabolism and potential
drug candidate for the treatment of type II diabetes and other metabolic
disorders. However, the half-life of active, circulating, human FGF-21 has
recently been shown to be limited in mice and monkeys by a proteolytic cleavage
between P171 and S172. Read More

Source: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0151269

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NIH Study: EagleBio’s HVA ELISA Assay

by Eagle Biosciences

Homovanillic Acid (HVA) ELISA is for Research Use Only. Not for diagnostic procedures.

 

EagleBio’s Homovanillic Acid (HVA) ELISA was recently utilized in an study by National Institute of Neurological Disorders and Stroke.

 

Peptide TFP5/TP5 derived from Cdk5 activator P35 provides neuroprotection in the MPTP model of Parkinson’s disease

Parkinson’s disease (PD) is a chronic neurodegenerative disorder characterized by the loss of dopamine neurons in the substantia nigra, decreased striatal dopamine levels, and consequent extrapyramidal motor dysfunction. Recent evidence indicates that cyclin-dependent kinase 5 (Cdk5) is inappropriately activated in several neurodegenerative conditions, including PD. Read more

(Source: https://www.molbiolcell.org/content/26/24/4478.short)

Related News:

Catecholamine Sensitive Assays: Product Highlights

EagleBio’s Serotonin ELISA: A Rock Solid Assay and 5 Recent Citations you Should Not Miss

New Study Referencing EagleBio’s Serotonin ELISA Assay Kit

January 21, 2015- Eagle Biosciences Shares Some New Publications Across Multiple EagleBio Assay Kit Product Lines

July 5, 2012: Eagle Biosciences Introduces High Sensitive Catecholamine Assay Kits

Related Kits:

Adrenaline ELISA

Dopamine ELISA

Noradrenaline ELISA

Neurobiology Assay Kits

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Serotonin may mediate effects of infection in the womb

by Eagle Biosciences


Infection during pregnancy blunts development of the fetal brain by boosting levels of the chemical messenger serotonin, a new study suggests(1).

A wealth of data tie infection in a pregnant woman to a slightly increased risk of autism in her child. Prenatal exposure to inflammation is known to disrupt brain development and produce autism-like behaviors in mice. Read More

Source: (https://spectrumnews.org/news/serotonin)

References (used in source above):
  1. Goeden N. et al. J. Neurosci. 36, 6041-6049 (2016) PubMed

 

Related Assays:

SEROTONIN ELISA ASSAY KIT

SEROTONIN ULTRASENSITIVE ELISA ASSAY KIT

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Maryland Study: EagleBio’s IGF-1 BIOTINYLATED (BIOTIN IGF-1)

by Eagle Biosciences
Maryland Study: EagleBio’s IGF-1 BIOTINYLATED (BIOTIN IGF-1)

EagleBio_Biotin-IGF1_Cancer_Research_Study

IGF-1 BIOTINYLATED (BIOTIN IGF-1) is for Research Use Only. Not for diagnostic procedures.

 

Eagle Biosciences’ IGF-1 BIOTINYLATED (BIOTIN IGF-1 was recently utilized in a study conducted at Frederick National Laboratory for Cancer Research in Maryland. Our Biotin- R3 IGF-I is prepared by a selective biotinylation procedure and is offered in a stable and convenient lyophilized solution. Our technology permits biotinylation without loss of binding capacity to
IGFBP´s or IGF antibodies. Potential Applications: Western-ligand blotting,Western-ligand blots with biotinylated IGF-I and analogs, and ELISAs.

 

Publication:

Mammalian cell transient expression, non-affinity purification, and characterization of human recombinant IGFBP7, an IGF-1 targeting therapeutic protein

It has been well documented that insulin-like growth factor 1 (IGF-
1) plays multiple roles during cancer development. Therefore, targeted
manipulation of IGF-1 signal pathway is an attractive strategy for cancer
treatment. Insulin-like growth factor-binding protein 7 (IGFBP7), a nonIgG
protein, interacts directly with IGF-1 and VPS24. Read More

(Source:https://www.researchgate.net/publication/282944657)

Related kits:

MULTISPECIES IGF-1 ELISA

Related News:

Cancer treatment on a cellular level

October 1, 2012: Eagle Biosciences Introduces Recombinant Human Insulin, Biotin-labeled (Biotinylated Insulin)

 

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Circadian and Melatonin Disruption by Exposure to Light at Night Drives Intrinsic Resistance to Tamoxifen Therapy in Breast Cancer

by Eagle Biosciences

Resistance to endocrine therapy is a major impediment to successful treatment of breast cancer. Preclinical and clinical evidence links resistance to antiestrogen drugs in breast cancer cells with the overexpression and/or activation of various pro-oncogenic tyrosine kinases. Disruption of circadian rhythms by night shift work or disturbed sleep-wake cycles may lead to an increased risk of breast cancer and other diseases. Moreover, light exposure at night (LEN) suppresses the nocturnal production of melatonin that inhibits breast cancer growth. Read More

Source:(https://cancerres.aacrjournals.org/content/74/15/4099)

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Write a Review for EagleBio Products and Earn a $25.00 Amazon Gift Card

by Eagle Biosciences
Write a Review for EagleBio Products and Earn a $25.00 Amazon Gift Card

Eagle Biosciences is a leading provider of immunoassays, antibodies, and proteins worldwide. We bring unique and solid life science test solutions with our Eagle developed products and products from our European partners. We strive to continually offer esoteric and innovative scientific tools that aid medical research and clinical laboratories. As the work of our customers continually evolves, our robust assay kits enable them to advance research in a variety of emerging areas of study and disciplines.

There have been several publications to date for our assays and if you are one of the many customers who has success and liked Eagle Biosciences’ products, you now have the chance to share your experience with other researchers on Biocompare.com,
and receive a $25 Amazon gift card. Learn more

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Interesting New Research: Team develops promising Zika vaccine candidates

by Eagle Biosciences


Researchers around the world have been racing to develop a vaccine for the Zika virus, and it appears that a large team of US researchers have developed two very promising candidates.

According to a report published in the journal Nature, researchers developed one potential vaccine using a Zika virus isolated in Brazil, and another candidate using a Zika virus isolated in Puerto Rico. Preclinical results showed both candidates were able to protect mice against an infection with the Zika virus by stimulating the rodents’ immune system. Furthermore, neither candidate was found to have significant side effects. Read More


Source: (https://www.redorbit.com/news/health/1113414773/promising-zika-vaccine-candidates-062816)