IFN-beta ELISA Assay Kit
The IFN-beta ELISA Assay Kit is For Research Use Only
Size: 1×96 wells
Sensitivity: 0.15 ng/ml
Dynamic Range: 0.312-20 ng/ml
Incubation Time: 3.5 hours
Sample Type: Serum, Plasma, Cell culture Supernatants
Sample Size: 100 µL
Alternative Names: Interferon beta, IFN-β, Human IFN-beta
Specificity: This assay recognizes natural and recombinant Human IFN-beta. This kit might cross-react to mouse and monkey IFN beta.
Assay Background
IFN-beta is a cytokine that belongs to the interferon family of signaling proteins, which are released as part of the innate immune response to pathogens. The protein encoded by this gene belongs to the type I class of interferons, which are important for defense against viral infections. In addition, type I interferons are involved in cell differentiation and anti-tumor defenses. Following secretion in response to a pathogen, type I interferons bind a homologous receptor complex and induce transcription of genes such as those encoding inflammatory cytokines and chemokines. Overactivation of type I interferon secretion is linked to autoimmune diseases. Mice deficient for this gene display several phenotypes including defects in B cell maturation and increased susceptibility to viral infection.
Related Products
Interferon Gamma ELISA Assay
Human High Sensitive IFN-gamma ELISA Assay
IFN Assay Type I Ready Cells
Assay Principle
This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for Human IFN-beta has been pre-coated onto a microtiter plate. Standards or samples are pipetted into the wells and any Human IFN-beta present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-conjugated antibody specific for Human IFN-beta is added to each well and incubate. Following a washing to remove unbound substances, streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After washing away any unbound antibody-enzyme reagent, a substrate solution (TMB) is added to the wells and color develops in proportion to the amount of Human IFN-beta bound in the initial step. The color development is stopped by the addition of acid and the intensity of the color is measured at a wavelength of 450nm ±2nm.The concentration of Human IFN-beta in the sample is then determined by comparing the O.D of samples to the standard curve.
Assay Procedure
1. Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal it.
2. Add 100 μl of standards, samples and zero controls (standard diluent buffer) into wells. Incubate for 1.5 h at 37°C.
3. Aspirate each well and wash, repeating the process four times for a total five washes. Wash by filling each well with 1× Wash Buffer (350 μl) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating, decanting or blotting against clean paper towels.
4. Add 100 μl 1X Antibody conjugate into each well. Cover wells and incubate for 1 hour at 37°C.
5. Aspirate each well and wash as step 3.
6. Add 100 μl of 1X HRP-Streptavidin solution to each well. Cover wells and incubate for 30 minutes at 37°C.
7. Aspirate each well and wash as step 3.
8. Add 100 μl of TMB Reagent to each well. Incubate for 15 minutes at 37°C in dark.
9. Add 100 μl of Stop Solution to each well. The color of the solution should change from blue to yellow.
10. Read the OD with a microplate reader at 450nm immediately.
Package Inserts
Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.
