Gastrointestinal Assay Kit

Clostridium Difficile GDH ELISA Assay Kit

$435.00

The C. Difficile GDH ELISA Assay Kit is intended for the quantitative and qualitative detection of C. difficile glutamate dehydrogenase 1 (GDH) in feces. For Research Use Only.

SKU: CDF35-K01 Categories: , ,

Clostridium Difficile GDH ELISA Assay Kit

Clostridium Difficile GDH ELISA Assay Kit Developed and Manufactured in the USA

Size: 1×96 Wells
Sensitivity: Cut-Off Control
Incubation Time: 2 hours
Sample Type: Stool
Sample Size: 250 mg
Alternative Names: C. Difficile, Fecal C. Difficile, Fecal Clostridium Difficile GDH, C. diff. Assay, C. diff ELISA, glutamate dehydrogenase
For Research Use Only

Controls Included

Assay Principle

This Fecal Clostridium Difficile (C.diff) GDH ELISA is a “sandwich” ELISA that is designed, developed and produced for the quantitative and qualitative measurement of GDH in stool specimen. The assay utilizes the microplate-based enzyme immunoassay technique by coating highly purified antibody onto the wall of microtiter wells.

Assay calibrators/controls and fecal specimen are added to microtiter wells of microplate that was coated with a highly purified monoclonal anti-GDH on its wall. During the assay, the GDH Antibody will be bound to the antibody coated plate after an incubation period. The unbound material is washed away and another HRP-conjugated monoclonal antibody which specifically recognizes the protein of GDH is added for further immunoreactions.  After an incubation period, the immunocomplex of “Anti-GDH Capture Antibody – GDH – HRP-conjugated Anti-GDH Tracer Antibody” is formed if GDH is present in the test sample. The unbound tracer antibody and other proteins in buffer matrix are removed in the subsequent washing step. HRP conjugated tracer antibody bound to the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the tracer antibody bound to GDH proteins captured on the wall of each microtiter well is directly proportional to the amount of GDH level in each test specimen.

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Clostridium Difficile Toxin AB Qualitative ELISA Assay Kit

Additional Information

Assay Background

Clostridium difficile is a gram-positive anaerobe. Infection with C.  difficile causes severe diarrhea and can be fatal if not diagnosed and treated in a timely manner. C. difficile infection is induced in patients by long-term treatment with antibiotics and is commonly found in hospital environment.  It is easily transmitted through contact with infected fecal matter. Accurate testing for toxins proved to be difficult due to assays having low sensitivity. All strains of C. difficile produce large amounts of glutamate dehydrogenase.

Specificity

The assay does not cross react to the following organisms: Cryptosoridium parvum, Giardia lamblia, rotavirus and adenovirus.

Package Inserts

 

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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