Clostridium Difficile Toxin A Qualitative ELISA Assay Kit

$340.00

The Eagle Biosciences Fecal C. Difficile Toxin A ELISA Assay Kit is a microplate-based ELISA (enzyme linked immunosorbent assay) and is intended for the qualitative detection of C. difficile Toxin A in feces. The assay is a useful tool as an aid of detection of C. difficile infection. This Eagle Biosciences Fecal C. Difficile Toxin A ELISA Assay Kit is for Research Use Only and is not intended for diagnostic or therapeutic purposes.

SKU: DFA35-K01 Categories: , ,

Clostridium Difficile Toxin A Qualitative ELISA Assay Kit

Size: 1×96 wells
Sensitivity: Cut-Off Control
Incubation Time: 2 hours
Sample Type: Stool
Sample Size: 250 mg

Controls Included

Product Developed and Manufactured in the USA


Additional Information

Assay Background


Clostridium difficile is a common pathogen and a major cause of infectious diarrhea in hospitalized patients. While most strains produce both Toxin A and Toxin B, some only produce one or the other. These enterotoxins, which are both proinflamatory and cytotoxic, attack the mucosal lining of the intestines. If not identified and treated in a timely fashion, can result in permanent damage to the colon or colitis. This assay employs an antibody specific to Toxin A without cross-reactivity to toxin B.

Assay Principle


This Eagle Biosciences Fecal C. Difficile Toxin A ELISA Assay Kit is a “sandwich” ELISA is designed, developed and produced for the qualitative measurement of Toxin A in stool specimen. The assay utilizes the microplate-based enzyme immunoassay technique by coating highly purified antibody onto the wall of microtiter wells.  Controls and extracted fecal specimen are added to microtiter wells of microplate that was coated with a highly purified monoclonal anti-Toxin A on its wall. During the assay, the Toxin A Antibody will be bound to the antibody coated plate after an incubation period. The unbound material is washed away and another HRP-conjugated monoclonal antibody which specifically recognizes the protein of Toxin A is added for further immunoreactions.  After an incubation period, the immunocomplex of “Anti-Toxin A Capture Antibody – C. Diff. Toxin A – HRP-conjugated Anti-Toxin A Tracer Antibody” is formed if Toxin A is present in the test sample. The unbound tracer antibody and other proteins in buffer matrix are removed in the subsequent washing step. HRP conjugated tracer antibody bound to the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the tracer antibody bound to Toxin A proteins captured on the wall of each microtiter well is directly proportional to the amount of Toxin A level in each test specimen.

Specificity


The assay does not cross react to the following: Toxin B, Helicobacter pylori, glutamate dehydrogenase 1 (GDH) Cryptosoridium parvum, Giardia lamblia, rotavirus and adenovirus.

Manual

Product Manual