Human Recombinant CDNF Protein
The Human Recombinant CDNF Protein is For Research Use Only
Synonyms: Cerebral dopamine neurotrophic factor – CDNF; ARMETL1
Uniprot ID: Q49AH0
Source: Human
Host: CHO-based cell line (expressed by QMCF Technology)
Buffer: PBS pH 7.4
Endotoxine: Less than 1EU/mg of protein as determined by LAL method
Molecular Weight:
Approximately 18.5 kDa, a single non-glycosylated polypeptide chain containing 163 amino acids
Purification:
Purified by ion-exchange chromatography and gel-filtration from serum-free CHO growth media. Protein is sterile-filtrated through 0.22 µg filter.
Concentration:
1 mg/ml Concentration of the protein is determined by BCA Protein Analysis kit (Pierce). BSA was used as a standard.
Bioproperties:
Protein is biologically active. Suitable for cell culture and in vivo testing. Biological activity of human CDNF was tested in rat 6-OHDA model of Parkinson’s disease and shown to be both neuroprotective and neurorestorative (for methods see: Lindholm et al. 2007; Voutilainen et al. 2011).
Quality Control:
SDS-PAGE and Western-Blot analysis (Figure 1 and 2, respectively). Mass-spectroscopy: Purified human CDNF is characterized as homogenous material by mass-spectroscopy.
Background:
CDNF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- (Lindholm et al. 20007; Voutilainen et al., 2011) and MPTP-induced degeneration (Airavaara et al., 2012) of dopamine neurons in rodent models of Parkinson’s disease. When administered after 6-OHDA or MPTP –lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta.
Shipping:
Shipped in dry-ice
Storage:
Store at -70°C upon receipt. Recommended to aliquot into smaller quantities.
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Product Developed and Manufactured by Icosagen
