CXCL12 SDF-1 Alpha ELISA Assay
The CXCL12 SDF-1 Alpha ELISA Assay is For Research Use Only (RUO)
Size: 1×96 wells
Sensitivity: 0.31 ng/ml
Dynamic Range: 0.625 – 40 ng/ml
Incubation Time: 2.5 hours
Sample Type: Serum, Plasma
Sample Size: 50 µL
Alternative Names: Stromal Cell-Derived Factor 1, C-X-C motif chemokine 12
Assay Background
SDF-1 Alpha (CXCL12) is a CXC chemokine [1]. The small basic protein (ca. 8 kDa) has no glycosylation site in its sequence [2]. The homology properties of SDF-1 Alpha are unique among the chemokines. It has a high degree of conservation within the mammalians, with 99 % identity between the human and the murine SDF-1 Alpha sequences. But it shows a weak homology to the other CXC chemokines and the SDF-1 gene is on chromosome 10, whereas the other chemokine genes are on chromosome 4 and 17 [3]. Except for SDF-1 Alpha there is another isoform of this chemokine, SDF-1 Beta. SDF-1 Alpha and SDF-1 Beta result from alternative splicing [1].SDF-1 Alpha binds to and activates the receptors CXCR4 and CXCR7 [1], whereupon SDF-1 Alpha is the only known ligand for CXCR4 [2]. SDF-1 Alpha binds also to fibronectin-1 [4], several glycosaminoglycans and heparin [5]. SDF-1 Alpha is constitutively expressed by bone marrow stromal cells [1] and many other tissues (for example heart, liver, brain, muscle, kidney) [3]. The function of SDF-1 Alpha is multifaceted. It plays a role in the prenatal development of the neural, hematopoietic and cardiovascular systems and is involved in the postnatal migration of CXCR4- and CXCR7-positive stem cells [1]. Furthermore, it has a chemo-attractant effect on CXCR4-positive lymphocytes [1] and plays a role in the growth and the metastasis of CXCR4- and CXCR7-positive tumors [6]. Except for the chemo-attractant effects SDF-1 Alpha has an inhibiting effect on HIV-1 in vitro [7].
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Product Developed and Manufactured in Germany by ibt – immunological and biochemical testsystems GmbH
