11,12 EET DHET ELISA Assay Kit
The 11,12 EET DHET ELISA Assay Kit is For Research Use Only
Size: 1×96 wells
Sensitivity: 0.01 ng/ml
Dynamic Range: 0.01 – 1000 ng/ml
Incubation Time: 2.5 hours
Sample Type: Serum, Plasma, Tissue, Biological Fluids
Sample Size: 100 µl
Product manufactured in the USA
Storage and Stability
The 11,12-EET/DHET Assay kit will obtain optimal results if all of the components are stored at the proper temperature prior to use. Items should be stored at the designated temperatures upon receipt of the 11,12-EET/DHET ELISA. All components are stored below -20°C and should not be re-frozen and thawed more than necessary.
EET+DHET can be measured after chemically changing EET to DHET. However, if the EET in cells or in blood is changed to DHET by abundantly expressed soluble epoxide hydrolase, measurement of DHET without chemically changing EET to DHET is suitable.
However, when P450 2C23 activity of the rat microsomes was measured, the rat microsomes were incubated with arachidonic acid (substrate of P450 2C23) and, then, EET + DHET levels in the reaction mixture were measured after acid hydrolysis of EET to DHET, which was indicative of P450 2C23 activity.
There are three different protocols which can be used to convert EET into DHET for measurement using the competitive ELISA kit. For optimal results please choose the protocol which fits your sample best.
It is well known that arachidonic acid (AA) will be converted to EET by P450 arachidonic acid epoxygenase (AA epoxygenase) and EET will be converted to DHET by soluble epoxide hydrolase (sEH) in vivo. Cytochrome P450 2J2 (CYP2J2) is a predominant human AA epoxygenase that produces all four EETs. The EET/DHET ELISA Assay kit can be used to measure EET levels in cultured cells which express sEH (1).