Human IGFBP-1
The Eagle Bioscience’s Human IGFBP-1 is for research use only and not to be used for diagnostic procedures.
Product Specifications
Source:
Purified from human amniotic fluid of individuals that have been shown by certified tests to be negative for hepatitis.
Description:
Human Insulin-like growth factor binding protein-1 (IGFBP-1) is a 234 amino acid protein which is a member of a family of six circulating proteins that bind IGF-I and IGF-II with high affinity and modulate their metabolic and mitogenic effects. The liver, ovarian granulosa cells, decidualized endometrium and a number of other cell types produce native human IGFBP-1. IGFBP-1 binds both IGF-I and IGF-II. Serum IGFBP-1 levels are regulated by insulin-induced inhibition of IGFBP-1 production. IGFBP-1 appears to inhibit smooth muscle migration in response to IGF-I or IGF-II and to stimulate wound healing in response to IGF-II. Native IGFBP-1 is predominantly a lower molecular weight species of 218 amino acids and 3-4 phosphorylated forms.
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- Purity: >90 % (by HPLC analysis)
- Quantity: 20 µg
- Molecular Weight: 25,300 Daltons – confirmed by Mass Spectrometry
- Biological Activity: Inhibition of IGF-I stimulated proliferation of NUC-1 cells: IC50 ~100 ng/ml. Inhibition of serum-induced stimulation of DNA synthesis in chick embryo fibroblast primary cultures: IC50 ~300 ng/ml
- Endotoxin (LAL): <0.1 EU/ug
- Storage/Stability: At least 2 years at 2-4°C (lyophilized)
- Appearance: White powder
- State: Freeze-dried from 10 mM HCl and stored under nitrogen under a slight vacuum.
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