GLP-1 (9-36) amide Control Set
The GLP-1 (9-36) amide Control Set is For Research Use Only
SIZE: 3 vials, 0.5 mL after reconstitution
Assay Background
Glucagon-like-peptide-1, GLP-1, is one of the major incretin hormones produced in the intestinal L-cells. It is secreted as the active forms (7-37)/(7-36) amide but is rapidly cleaved by the enzyme DPP4 forming the metabolites GLP-1 (9-37)/(9- 36) amide1 . Although the two active forms have shown equal biological potency, the majority of circulating GLP-1 in humans is found in the amidated form. It is also the amidated forms that increase significantly after a meal, keeping the nonamidated forms at a more basal level. In order to study GLP-1 secretion in vivo it is necessary to measure the metabolite (9-36) amide since only small amounts of the intact hormone will be found in the circulation. GLP-1 is released post-prandially in the intestine and has the ability to increase glucose-stimulated insulin secretion and inhibit glucagon secretion. The dual influence that GLP-1 exerts on pancreatic function has made it an attractive target for pharmacological interventions, giving rise to the development of peptide mimetics and inhibitors of DPP.
Preparation of reagents
Reconstitute each GLP-1 (9-36) amide Control (Low, Medium, High) by adding 500 µL redistilled water to each vial. Replace the rubber stopper and allow to stand for 5 minutes. Gently swirl until contents are thoroughly mixed. Avoid foaming.
Shelf life and storage
The expiry date is stated below. Store the complete package at 2-8°C. Reconstituted controls are stable for 2 months at
-20°C. Avoid repeated freeze/thaw cycles and storage at 2-8°C or room temperature
