Total GLP-1 NL-ELISA Assay Kit
The Total GLP-1 NL-ELISA Assay Kit was manufactured in Sweden by Mercodia
Size: 12 x 8 wells
Sensitivity: 0.65 pmol/L
Dynamic Range: 1 – 940 pmol/L
Incubation Time: 2 hours 15 minutes
Sample Type: Serum, EDTA plasma
Sample Size: 25 µL
Glucagon-like-peptide-1, GLP-1, is one of the major incretin hormones produced in the intestinal L-cells. It is secreted as the active forms (7-37)/(7-36) amide but is rapidly cleaved by the enzyme DPP4 forming the metabolites GLP-1 (9-37)/(9- 36) amide1 . Although the two active forms have shown equal biological potency, the majority of circulating GLP-1 in humans is found in the amidated form. It is also the amidated forms that increase significantly after a meal, keeping the nonamidated forms at a more basal level. In order to study GLP-1 secretion in vivo it is necessary to measure the metabolite (9-36) amide since only small amounts of the intact hormone will be found in the circulation. GLP-1 is released post-prandially in the intestine and has the ability to increase glucose-stimulated insulin secretion and inhibit glucagon secretion. The dual influence that GLP-1 exerts on pancreatic function has made it an attractive target for pharmacological interventions, giving rise to the development of peptide mimetics and inhibitors of DPP.
The Total GLP-1 NL-ELISA Assay Kit is a solid phase two-site enzyme immunoassay based on the sandwich technique, in which two monoclonal antibodies are directed against separate antigenic determinants on the GLP-1 molecule. GLP-1 in the sample reacts with anti-GLP-1 antibodies bound to microtitration wells and peroxidase-conjugated anti-GLP-1 antibodies in the solution. A simple washing step removes unbound enzyme-labelled antibody. The bound conjugate is detected by reaction with the chemiluminescent substrate. A luminescence plate reader is used to read the intensity of light generated.