Total GLP-1 NL-ELISA Assay Kit


The Total GLP-1 NL-ELISA Assay Kit provides a method for the quantitative determination of amidated GLP-1 in human plasma and serum samples. The Total GLP-1 NL-ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

SKU: 10-1278-01 Categories: , ,

Total GLP-1 NL-ELISA Assay Kit

The Total GLP-1 NL-ELISA Assay Kit was manufactured in Sweden by Mercodia

Size: 12 x 8 wells
Sensitivity: 0.65 pmol/L
Dynamic Range: 1 – 940 pmol/L
Incubation Time: 2 hours 15 minutes
Sample Type: Serum, EDTA plasma
Sample Size: 25 µL

Assay Background

Glucagon-like-peptide-1, GLP-1, is one of the major incretin hormones produced in the intestinal L-cells. It is secreted as the active forms (7-37)/(7-36) amide but is rapidly cleaved by the enzyme DPP4 forming the metabolites GLP-1 (9-37)/(9- 36) amide1 . Although the two active forms have shown equal biological potency, the majority of circulating GLP-1 in humans is found in the amidated form. It is also the amidated forms that increase significantly after a meal, keeping the nonamidated forms at a more basal level. In order to study GLP-1 secretion in vivo it is necessary to measure the metabolite (9-36) amide since only small amounts of the intact hormone will be found in the circulation. GLP-1 is released post-prandially in the intestine and has the ability to increase glucose-stimulated insulin secretion and inhibit glucagon secretion. The dual influence that GLP-1 exerts on pancreatic function has made it an attractive target for pharmacological interventions, giving rise to the development of peptide mimetics and inhibitors of DPP.

Assay Principle

The Total GLP-1 NL-ELISA Assay Kit is a solid phase two-site enzyme immunoassay based on the sandwich technique, in which two monoclonal antibodies are directed against separate antigenic determinants on the GLP-1 molecule. GLP-1 in the sample reacts with anti-GLP-1 antibodies bound to microtitration wells and peroxidase-conjugated anti-GLP-1 antibodies in the solution. A simple washing step removes unbound enzyme-labelled antibody. The bound conjugate is detected by reaction with the chemiluminescent substrate. A luminescence plate reader is used to read the intensity of light generated.

Related Products

Solid Phase Sample Extraction Kit
Mouse/Rat GLP-1 Active (7-36) ELISA Assay Kit

Additional Information

Standard Curve

Package Inserts

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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