Zika Virus IgM ELISA Assay Kit

$515.00

The Zika Virus IgM ELISA Assay Kit is a microplate-based ELISA (enzyme linked immunosorbent assay) kit intended for the qualitative detection of Zika virus IgM antibodies in blood sample. The assay is useful as an aid in determining recent infection by Zika virus. The Eagle Biosciences Zika Virus IgM ELISA Assay Kit is for research only and not used for diagnostic procedures.

SKU: ZIK31-K01 Categories: , ,

Zika Virus IgM ELISA Assay Kit

Zika Virus IgM ELISA Assay Kit is developed and manufactured in the USA

Size: 1×96 wells
Incubation Time:  >2 hours
Sample Type:  Serum or Plasma
Sample Size: 10 µL
For Research Use Only


Assay Principle

The Zika Virus IgM ELISA is a “sandwich” ELISA is designed, developed and produced for the qualitative measurement of IgM antibodies in blood specimen. The assay utilizes the microplate-based enzyme immunoassay technique by coating highly purified antigen onto the wall of microtiter wells. Controls and blood specimen are added to microtiter wells of microplate that is coated with highly specific anti human IgM antibodies on its wall. During the assay, Zika antibodies will be bound to the antibody coated plate after an incubation period. The unbound material is washed away and biotinylated Zika antigen is added for further immunoreactions. The unbound antigen is washed away and a highly specific monoclonal antibody which is labeled with horseradish peroxidase is added to the wells. After an incubation period, the immunocomplex of “anti human IgM – Zika virus – HRP-conjugated anti Zika virus Tracer Antibody” is formed if Zika IgM antibodies are present in the test sample. The unbound tracer antibody and other proteins in buffer matrix are removed in the subsequent washing step. HRP conjugated tracer antibody bound to the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the tracer antibody bound to Zika IgM antibodies captured on the wall of each microtiter well is directly proportional to the amount of Zika IgG antibody level in each test specimen.


Products Related to Zika Virus IgM ELISA

Zika Virus IgG ELISA Assay Kit
Dengue Virus IgM Antibody ELISA Assay Kit
Human Anti-Giardia Iamblia IgM ELISA Assay Kit


Related News

Zika Virus Could Potentially be Used to Kill Brain Cancer Stem Cells (Original Article)
Zika Virus Infection in Mice Causes Panuveitis with Shedding of Virus in Tears

Additional Information

Assay Background


Zika virus is a flavivirus, in the family Flaviviridae. Zika was discovered in Uganda and has spread rapidly to the Americas and the Caribbean in the past year. Zika virus is mainly spread by the bite of infected mosquitos (Aedes aegypti and Aedes albopictus). Zika virus can also be transmitted from person to person through sexual contact, by blood transfusion or contact with infected materials. The infection usually results in mild or sometimes no symptoms and is normally not life threatening. Women can transmit the virus to the fetus during pregnancy. Infection during pregnancy is deleterious to the fetus and is associated with fetal death, fetal growth restriction, and a spectrum of central nervous system abnormalities.

Assay Procedure


  1. Place a sufficient number of microplate strips in a holder to run assay controls and desired unknown samples in duplicate. See test configuration for example.
  2. Dispense 100 μL of Negative Control, diluted (1:100) positive control into respective wells.
  3. Carefully mix the plate by tapping side gently for 5-10 seconds, making sure not to splash.
  4. Cover the plate with aluminum foil to avoid exposure to light. Incubate the plate at 37°C for 30 minutes.
  5. Wash each well 5 times by dispensing 350 µl of working wash solution into each well and then completely aspirating the contents. Alternatively, an automated microplate washer can be used.
  6. Add 100 µL biotinylated Zika virus  Antigen (31016) into each well.
  7. Cover the plate with an aluminum foil to avoid exposure to light. Incubate the plate at ROOM TEMPERATURE for 30 minutes.
  8. Wash each well 5 times by dispensing 350 µl of working wash solution into each well and then completely aspirating the contents. Alternatively, an automated microplate washer can be used.
  9. Add 100 µL HRP Conjugated Streptavidin (31017) into each well.
  10. Cover the plate with an aluminum foil to avoid exposure to light. Incubate the plate at ROOM TEMPERATURE for 30 minutes.
  11. Add 100 µl of ELISA HRP Substrate into each well.
  12. Cover the plate with an aluminum foil to avoid exposure to light. Incubate plate at room temperature for 20 minutes
  13. Add 100 µl of ELISA Stop Solution into each of the wells. Mix gently by tapping, making sure not to splash.
  14. Read the absorbance at 450 nm immediately using a microplate reader.

Documents

Product Documents


 

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

Product Citations