Ustekinumab mAb-based ELISA Assay
The Ustekinumab mAb-based ELISA Assay is For Research Use Only
Size: 1 x 96 wells
Sensitivity: 1.5 ng/mL
Dynamic Range: 2-60 ng/mL
Incubation Time: 2.5 hours
Sample Type: Serum, Plasma
Sample Size: 10 µL
Alternative Name: Stelara
The Ustekinumab mAb-based ELISA is based on Ustekinumab-specific monoclonal antibody (catcher Ab, ImmunoGuide clone 9C7). Diluted standards and samples are incubated in the microtiter plate coated with IG-9C7 mAb. After incubation, the wells are washed. A biotinylated anti-human IgG monoclonal antibody (clone IG-1B5; specific for the Fc part of all human IgG i.e. IgG1, IgG2, IgG3 and IgG4) is added and binds to the Fc part of Ustekinumab. Following incubation, wells are washed and the horseradish peroxidase (HRP)-conjugated streptavidin is added and binds to the biotinylated 1B5 mAb. Following incubation, wells are washed and the bound enzymatic activity is detected by addition of chromogen-substrate. The color developed is proportional to the amount of Ustekinumab in the sample or standard. Results of samples can be determined by using the standard curve. Preincubation of Ustekinumab with recombinant human interleukin-12 (IL-12), contains p40 protein subunit as that of IL-23, inhibited the reaction. Therefore, the Eagle Biosciences Ustekinumab ELISA (mAb-based) measures the free form of Ustekinumab.
Storage and Stability of the Ustekinumab mAb-based ELISA Assay
The kit is shipped at ambient temperature and should be stored at 2-8°C. Keep away from heat or direct sun light. The microtiter strips are stable up to the expiry date of the kit in the broken, but tightly closed bag when stored at 2–8°C.