Ubiquinone Coenzyme Q10 HPLC Assay

$780.00

The Ubiquinone Coenzyme Q10 HPLC Assay is intended for the quantitative determination of ubiquinone (coenzyme Q10) in plasma, serum and EDTA-blood. The Ubiquinone (Coenzyme Q10) HPLC Assay kit is for research use only and should not be used for diagnostic procedures.

Ubiquinone Coenzyme Q10 HPLC Assay

The Ubiquinone Coenzyme Q10 HPLC Assay is For Research Use Only

Size: 100 tests
Incubation Time: 10 minutes
Sample Type: Serum, Plasma, EDTA Blood
Sample Size: 200 µL
Reference Values (Normal):

  • EDTA-plasma: 0.8 – 1.4 µg/ml (Mortensen S. A. et al: (1997) Molec.Aspects Med. 18:137-144)

Developed and Manufactured in Germany
Product Support in USA

It is suggested that each laboratory establish its own normal ranges


Assay Background

Ubiquinone is a coenzyme of every cell in the body. It was first isolated in the 50th from Prof. Green from Wisconsin. For his investigations concerning the function Prof. Mitchel received 1978 the Nobel price. Ubiquinone is built up by a chinonic ring and an isoprenic sidechain. In humans ubiquinone can be synthesized and taken up by nutrition. Ubiquinone has two different pysiological functions.
1.) Component of the energy metabolism
During the reduction of oxygen in the oxydative phosphorylation electrons are tranferred from NADPH to oxygen via 6 different redox systems. Ubiquinone is the less abundant redox system in the membrane of the mitochondria. Because of the low amount it is the speed controlling redox-system in the energy metabolism. Normally the amount of ubiquinone is sufficient, but with growing age and exposure to sunlight it is reduced to 50 %.
2.) Radical scavanger
Ubiquinone has a high amount of carbon doublebonds and therefore a higher potential of reduction than vitamin C or vitamin E. Thereby it is the first line of defense against free radicals. Even when other antioxidants are inert coenzyme Q10 reacts fast and sensitive against free radicals. Therefore ubiquinone is an optimal stabilizer of the ion channels of the membranes.


Related Products

Ubiquinone / Coenzyme Q10 HPLC Assay Column
Ubiquinone / Coenzyme Q10 HPLC Assay Control Set
Vitamin C HPLC Assay Kit

Additional Information

Assay Principle


For the determination of ubiquinone (coenzyme Q10) a precipitation step which removes high molecular substances is performed first. The internal standard reduces the variation in the sample treatment. After centrifugation the supernatant is mixed with an extraction solution and transferred to the organic phase. The organic solvent is evaporated and the sample is re-suspended in ethanol p.A. and injected into the HPLC system.  The isocratic separation via HPLC at 30°C using a “reversed phase“ column needs 15 minutes. The chromatograms are recorded by a UV-detector. The quantification is performed by the delivered calibrator and the concentration is calculated by the “internal standard method” via integration of the peak areas resp. peak heights.


Assay Background


Ubiquinone is a coenzyme of every cell in the body. Ubiquinone is made up by a chinonic ring and an isoprenic side chain. In humans ubiquinone can be synthesized and taken up by nutrition.  Ubiquinone has two different physiological functions.

  1. Component of the energy metabolism
    • During the reduction of oxygen in the oxidative phosphorylation, electrons are transferred from NADPH to oxygen via 6 different redox systems. Ubiquinone is the less abundant redox system in the membrane of the mitochondria. Because of the low amount it is the speed controlling redox-system in the energy metabolism. Normally the amount of ubiquinone is sufficient, but with growing age and exposure to sunlight it is reduced to 50 %.
  2. Radical scavenger
    • Ubiquinone has a high amount of carbon double bonds and therefore a higher potential of reduction than either vitamin C or vitamin E. Thereby it is the first line of defense against free radicals. Even when other antioxidants are inert, coenzyme Q10 reacts fast and sensitive against free radicals. Therefore ubiquinone is an optimal stabilizer of the ion channels of the membranes.

The Eagle Biosciences Ubiquinone (Coenzyme Q10) HPLC Assay kit makes it possible to determine ubiquinone in an easy, fast and precise method. The Ubiquinone (Coenzyme Q10) HPLC Assay kit includes all reagents ready to use for preparation and separation of the samples with exception of the column (IC1700rp) and the controls (IC1700ko).  Both can be supplied by Eagle Biosciences.  Besides the complete test kit, it is possible to order all components separately. Please request our single component price list.

Manual

Product Manual