Total GIP NL-ELISA Assay Kit

$595.00

The Total GIP NL-ELISA Assay Kit provides a method for the quantitative determination of total GIP in human samples. The Total GIP NL-ELISA Assay Kit is for research use only and not to be used for diagnostic procedures.

SKU: 10-1258-01 Categories: ,

Total GIP NL-ELISA Assay Kit

The Total GIP NL-ELISA Assay Kit was manufactured in Sweden by Mercodia

Size: 12 x 8 wells
Sensitivity: 1.62 pmol/L
Dynamic Range: 2.7 – 1000 pmol/L
Incubation Time: Overnight
Sample Type: Serum, EDTA plasma
Sample Size: 25 µL


Assay Background

Glucose dependent insulinotropic polypeptide (GIP) is an incretin, a gut-derived circulating peptide hormone that potentiates glucose-dependent insulin secretion following ingestion of a meal1. GIP(1-42) (active GIP) is rapidly cleaved in circulation at the N-terminus by DPP-4 to yield GIP(3-42), regarded as a non-active form of GIP2. Because of this quick proteolytic degradation, not only intact but also total (i.e. intact plus DPP-4-metabolized) forms of GIP must be measured to study its secretion and processing in vivo.

Assay Principle

The Total GIP NL-ELISA Assay Kit is a solid phase two-site enzyme immunoassay based on the sandwich technique, in which two monoclonal antibodies are directed against separate antigenic determinants on the GIP molecule. GIP in the sample reacts with anti-GIP antibodies bound to microtitration wells and peroxidase-conjugated anti-GIP antibodies in the solution. A simple washing step removes unbound enzyme-labelled antibody. The bound conjugate is detected by reaction with the chemiluminescent substrate. A luminescence plate reader is used to read the intensity of light generated.


Related Products

Active GIP ELISA Assay Kit
Rat GIP ELISA Assay Kit
Mouse GIP ELISA Assay Kit

Additional Information

Standard Curve

Documents

Product Manual


Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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